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  • C6344 - Monoclonal Anti-Connexin-32 antibody produced in mouse

C6344 Sigma-Aldrich

Monoclonal Anti-Connexin-32 antibody produced in mouse

clone CXN-32, ascites fluid, buffered aqueous solution



Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies to Adhesion Molecules and Related Proteins, Antibodies to Cell and Organelle Proteins,
conjugate   unconjugated
clone   CXN-32, monoclonal
biological source   mouse
application(s)   immunohistochemistry (frozen sections): suitable
  indirect ELISA: suitable
  microarray: suitable
  western blot: 1:1,000 using a mouse whole brain extract
species reactivity   mouse, human, rat
mol wt   antigen 27 kDa
form   buffered aqueous solution
shipped in   dry ice
storage temp.   −20°C
antibody form   ascites fluid
isotype   IgG1
Quality Level   200
antibody product type   primary antibodies
contains   15 mM sodium azide
UniProt accession no.   P08034
Gene Information   human ... GJB1(2705), GJB2(2706)
mouse ... Gjb1(14618)
rat ... Gja1(24392), Gjb1(29584)


General description

Monoclonal Anti-Connexin-32 (mouse IgG1 isotype) is derived from the CXN-32 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. The 27 kDa connexin protein (Connexin-32, Cx32), belongs to the connexion family of proteins. It is expressed in most tissues, even though the pattern of expression may differ in various cell types including peripheral and central nervous system.


synthetic connexin-32 peptide (amino acids 105-123).


Monoclonal Anti-Connexin-32 antibody produced in mouse has been used in:
• enzyme linked immunosorbent assay (ELISA)
• immunoblotting
• epifuorescent microscopy


Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biochem/physiol Actions

Connexin-32 low expression levels is observed in common bile duct ligation (CBDL). In addition, a combination of myelin disruption and axonal degeneration has been shown to occur with Cx32 mutations in Charcot-Marie-Tooth disease (CMTX). Monoclonal antibodies reacting specifically with Cx32, may be used in diverse cellular and molecular approaches to the study of gap junctions and their properties, and to correlate their expression pattern with physiological functions or pathological conditions.

Connexins belong to the gap junction protein family. In humans, connexin is encoded by GJB1 (gap junction protein, β 1) gene. The gap junction proteins connexin32 (Cx32), Cx37, Cx40 and Cx43 are expressed in endothelial cells and regulate vascular functions involving inflammation. The endothelial Cx32 positively regulates angiogenesis by enhancing endothelial cell tube formation and cell migration. CX32 mutation is associated with cognitive impairment and a differential degree of peripheral nerve involvement.

Safety & Documentation

Safety Information

NONH for all modes of transport
WGK Germany 
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable


Certificate of Analysis (COA)

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Protocols & Articles


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