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  • C6344 - Monoclonal Anti-Connexin-32 antibody produced in mouse

C6344 Sigma

Monoclonal Anti-Connexin-32 antibody produced in mouse

clone CXN-32, ascites fluid, buffered aqueous solution



Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies to Adhesion Molecules and Related Proteins, Antibodies to Cell and Organelle Proteins,
species reactivity   rat, human, mouse
application(s)   immunohistochemistry (frozen sections): suitable
  indirect ELISA: suitable
  microarray: suitable
  western blot: 1:1,000 using a mouse whole brain extract
clone   CXN-32, monoclonal
antibody form   ascites fluid
form   buffered aqueous solution
isotype   IgG1
mol wt   antigen mol wt 27 kDa
contains   15 mM sodium azide
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... GJB1(2705), GJB2(2706)
mouse ... Gjb1(14618)
rat ... Gja1(24392), Gjb1(29584)
biological source   mouse
conjugate   unconjugated



synthetic connexin-32 peptide (amino acids 105-123).


Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)

Monoclonal Anti-Connexin-32 antibody produced in mouse is suitable for the following applications:
• Immunohistochemistry
• Indirect ELISA
• Microarray
• Western blotting

Biochem/physiol Actions

Connexins belong to the gap junction protein family. In humans, connexin is encoded by GJB1 (gap junction protein, β 1) gene. The gap junction proteins connexin32 (Cx32), Cx37, Cx40 and Cx43 are expressed in endothelial cells and regulate vascular functions involving inflammation. The endothelial Cx32 positively regulates angiogenesis by enhancing endothelial cell tube formation and cell migration. CX32 mutation is associated with cognitive impairment and a differential degree of peripheral nerve involvement.

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Safety & Documentation

Safety Information

RIDADR  NONH for all modes of transport
WGK Germany  3
Protocols & Articles


Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Carcinogenesis and Epigenetics

Cancer research has revealed that the classical model of carcinogenesis, a three step process consisting of initiation, promotion, and progression, is not complete. The expansion of the carcinogenesi...
Vicki Caligur
BioFiles 2008, 3.5, 18.
Keywords: Acetylations, Adhesion, Alkylations, Apoptosis, Biofiles, Cancer, Carcinogens, Cell division, Cell proliferation, Cell signaling, DNA microarrays, Drug discovery, Environmental, Epigenetics, Events, Gene expression, Genetic, Hormones, Metabolism, Methylations, Microarray Analysis, Mutagens, PAGE, Recombination, Reductions, Sequences, Transcription, Type, transformation


Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Monoclonal and polyclonal primary antibodies are focused on cell biology, neurobiology and molecular biology. Secondary antibodies targeting multiple host’s IgG are conjugated to alkaline phosphatase...
Keywords: Amplification, Buffers, Cell biology, Enzyme-linked immunosorbent assay, Immunofluorescence, Immunohistochemistry, Inflammation, Molecular biology, Phosphorylations, Purification, Western blot

Peer-Reviewed Papers


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