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C6555 Sigma-Aldrich

Monoclonal Anti-CD45RO antibody produced in mouse

clone UCHL-1, purified immunoglobulin, buffered aqueous solution

Synonym: Monoclonal Anti-CD45RO

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Properties

Related Categories Alphabetical Index, Analytical Cytology, Antibodies, Antibodies for Cell Biology, Antibodies for Immune Cell Signaling and Blood,
conjugate   unconjugated
clone   UCHL-1, monoclonal
biological source   mouse
application(s)   flow cytometry: 10 μL using 1 × 106 cells
species reactivity   human
mol wt   antigen 180 kDa
form   buffered aqueous solution
shipped in   wet ice
storage temp.   2-8°C
antibody form   purified immunoglobulin
antibody product type   primary antibodies
UniProt accession no.   P08575
Gene Information   human ... PTPRC(5788)

Description

Specificity

Recognizes the CD45RO human cell surface glycoprotein. The epitope recognized by this antibody is sensitive to neuraminidase digestion, but not to formalin fixation and paraffin embedding. The antibody is capable of detecting CD45RO antigen in tissue sections processed with a variety of fixatives. The antibody also detects CD45RO antigen in frozen sections, cell suspension, smears, imprints and cytospins.
4th Workshop: code no. N31
5th Workshop: code no. T081, CD45.43

Immunogen

influenza virus activated CA1 T-cell line.

Application

Monoclonal Anti-CD45RO antibody produced in mouse is suitable for flow cytometry at a dilution of 10μL using 1 × 106 cells.

Target description

CD45RO is a single-chain transmembranous glycoprotein, which represents the low molecular weight isoform of the leukocyte common antigen (LCA). It is expressed on most thymocytes, about 45% of resting peripheral blood T cells, virtually all T cells in skin reactive infiltrates and the majority of T cell malignancies. It is also found on a subset of B cells and on some B cell lymphomas. NK-cells do not express this antigen.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biochem/physiol Actions

Protein tyrosine phosphatase, receptor type, C, known as PTPRC, is an enzyme encoded by the PTPRC gene in humans. CD45RO is N-glycosylated. Deglycosylation at N327 of CD45RO results in increased binding to galectin-3 without affecting apoptosis, while deglycosylation at N36 or N109 of CD45RO enhanced galectin-3-induced apoptosis. The low levels of CD45 expression are specific to chronic lymphocytic leukemia (CLL) cells and reflect cell fragility.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
2

Documents

Certificate of Analysis

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Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

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Peer-Reviewed Papers
15

References

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