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  • C6615 - Anti-phospho-c-Kit (SCFR) (pTyr730) antibody produced in rabbit

C6615 Sigma-Aldrich

Anti-phospho-c-Kit (SCFR) (pTyr730) antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

Synonym: Anti-phospho-SCF Receptor (pTyr730)



Related Categories Alphabetical Index, Antibodies, Antibodies against Proteins/Bioactives/Markers/Receptors for Stem Cell Biology, Antibodies for Cell Biology, Antibodies for Hematopoietic Stem Cells,
conjugate   unconjugated
clone   polyclonal
biological source   rabbit
application(s)   western blot: 0.1-1.0 μg/mL using M07e cells
species reactivity   rat, human, mouse
form   buffered aqueous solution
shipped in   dry ice
storage temp.   −20°C
antibody form   affinity isolated antibody
antibody product type   primary antibodies
UniProt accession no.   P10721
Gene Information   human ... KIT(3815)
mouse ... Kit(16590)
rat ... Kit(64030)


General description

Mast cells play an important role in innate immunity or the response of the body to invading pathogens. The functions of mast cells are regulated by a number of surface receptors; c-Kit (Stem cell factor receptor, SCF R; CD117) being the most critical. The signalling triggered by c-Kit results in mast cell activation in response to antigens. The response involves tyrosine phosphorylation by specific tyrosine kinases that drive the downstream signalling process. The secondary pathways activated by c-Kit include Akt, mTOR, MAPK, Sos-Grb2 and c-Jun. Phosphorylation of tyrosine 730 is required for the binding of PLCγ leading to activation of various signalling pathways In addition to development of mast cells, c-Kit also regulates their subsequent maturation and homing to the residence sites. Constitutive activation by SCF R is implicated in myeloproliferative discorders, systemic mastocytosis and gastrointestinal stomal cell tumors
The antibody detects human c-Kit pTyr730. Mouse, rat and cow (100% homologous), dog (92% homologous), chicken (85%) are expected to react.


synthetic phosphopeptide derived from the region of human c-Kit that contains tyrosine 730.


Anti-phospho-c-Kit (SCFR) pTyr730 antibody may be used for immunoblotting at a working concentration of 0.1-1.0 μg/ml using M07e cells.

Physical form

Solution in Dulbecco′s phosphate buffered saline (without Mg2+ and Ca2+), pH 7.3, with 1.0 mg/mL BSA (IgG and protease free) and 0.05% sodium azide.


Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Safety Information

WGK Germany 

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