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  • C6805 - Monoclonal Anti-Collagen, Type VII antibody produced in mouse

C6805 Sigma

Monoclonal Anti-Collagen, Type VII antibody produced in mouse

clone LH7.2, ascites fluid



Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Hematopoietic Stem Cells, Antibodies for Stem Cell Biology,
species reactivity   pig, goat, monkey, marmoset, bovine, guinea pig, sheep, human
application(s)   dot blot: suitable
  immunocytochemistry: suitable
  indirect ELISA: suitable
  indirect immunofluorescence: 1:1,000 using human or other mammalian frozen sections
  western blot: suitable
clone   LH7.2, monoclonal
antibody form   ascites fluid
isotype   IgG1
contains   15 mM sodium azide
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... COL7A1(1294)
biological source   mouse
conjugate   unconjugated



insoluble fractions of neonatal human foreskin epidermal cells

General description

Collagen is a fibrous protein present in the extracellular framework of all vertebrates. Collagen type VII is the crucial component of anchoring fibrils and mutation in type VII collagen gene (COL7A1) leads to epidermolysis bullosa dystrophica. Monoclonal anti-collagen, type VII antibody can be used to distinguish invasive melanoma from non-invasive melanoma by visualizing the appearance and integrity of epidermal basement membrane. It can also be used in immunoblotting. Mouse anti-collagen type VII antibody reacts specifically with an epitope located on collagenase digested type VII collagen (150 kDa). The product also reacts with the basement membrane zone of stratified squamous epithelia of sheep, pig, bovine, guinea pig, goat and human.


Monoclonal anti-collagen type VII antibody can be used as a primary antibody in virus titration. It can also be used for the localization of type VII collagen in different immunochemical assays like dot blot, immunocytochemistry and ELISA.

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Safety Information

RIDADR  NONH for all modes of transport
WGK Germany  2
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Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy


Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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