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C7104 Sigma-Aldrich

Anti-Cdc27 antibody,Mouse monoclonal

clone AF3.1, purified from hybridoma cell culture

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Cell Cycle, Antibodies to Cyclins and CDKs,
conjugate   unconjugated
clone   AF3.1, monoclonal
biological source   mouse
application(s)   immunocytochemistry: suitable using 4% paraformaldehyde fixation, Triton X-100 permeabilization
  immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
  immunoprecipitation (IP): suitable
  indirect ELISA: suitable
  microarray: suitable
  western blot: 1-2 μg/mL using a HeLa Cell nuclear extract
species reactivity   rat, bovine, human, canine, Xenopus, mouse
mol wt   antigen 97 kDa
form   buffered aqueous solution
shipped in   dry ice
storage temp.   −20°C
antibody form   purified from hybridoma cell culture
isotype   IgG2b
antibody product type   primary antibodies
concentration   ~2 mg/mL
UniProt accession no.   P30260
Gene Information   human ... CDC27(996)
mouse ... Cdc27(217232)
rat ... Cdc27(360643)

Description

General description

Monoclonal Anti-Cdc27 (mouse IgG2b isotype) is derived from the AF3.1 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from a BALB/c mouse immunized with a synthetic peptide corresponding to C-terminal of human Cdc27, conjugated to KLH.

The gene CDC27 (Cell division cycle protein 27 homolog) is mapped to human chromosome 17q12–23.2. The protein localizes in the nucleus.

Immunogen

synthetic peptide corresponding to the C-terminus 10 residues of human Cdc27.

Application

Monoclonal Anti-Cdc27 antibody produced in mouse is suitable for the following applications:
• Immunocytochemistry using 4% paraformaldehyde fixation and Triton X-100 permeabilization
• Immunohistochemistry (formalin-fixed, paraffin-embedded sections)
• Immunoprecipitation.
• Indirect ELISA
• Microarray
• Western blotting / immunoblotting at a concentration of 1-2μg/mL using a HeLa Cell nuclear extract.
• Anaphase-promoting complex/cyclosome APC/C Purification.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biochem/physiol Actions

CDC27 (Cell division cycle protein 27 homolog) is a component of anaphase-promoting complex/cyclosome (APC/C), an E3 ligase involved in cell cycle control. Phosphorylation of CDC27 is crucial for tumor growth factorβ (TGFβ)-induced activation of APC. It interacts with Microcephalin (MCPH1), causative gene for primary recessive autosomal microcephaly. DNA-damage response mediator MDC1 interacts with APC/C through CDC27. Down-regulation of CDC27 in irradiated cervical cancer cell line results in greater survival fraction. Polymorphism in CDC27 are linked with breast cancer.

The Cdc16/27/34 complex or the Anaphase-promoting complex/cyclosome (APC/C) complex functions as an ubiquitin conjugating enzyme, ubiquitinating cyclin B, and resulting in cyclin B/Cdk complex degradation.

Safety & Documentation

Safety Information

WGK Germany 
-

Documents

Certificate of Analysis

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Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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