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C7207 Sigma-Aldrich

Monoclonal Anti-β-Catenin antibody produced in mouse

clone 15B8, ascites fluid

Synonym: Monoclonal Anti-β-Catenin

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Properties

Related Categories AA - CA, Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Epigenetics and Nuclear Signaling,
conjugate   unconjugated
clone   15B8, monoclonal
biological source   mouse
application(s)   immunocytochemistry: suitable
  immunohistochemistry (frozen sections): suitable
  indirect immunofluorescence: 1:1000 using cultured MDBK cells
  microarray: suitable
  western blot: 1:1000 using cultured MDBK cells extract
species reactivity   human, bovine, chicken, canine
mol wt   antigen 94 kDa
shipped in   dry ice
storage temp.   −20°C
antibody form   ascites fluid
isotype   IgG1
Quality Level   200
antibody product type   primary antibodies
Featured Industry   Research Pathology
UniProt accession no.   P35222
Gene Information   human ... CTNNB1(1499)

Description

General description

Monoclonal Anti-β-Catenin (mouse IgG1 isotype) is derived from the 15B8 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice. β-catenin belongs to the armadillo family and comprises the N-terminal region with 12 imperfect repeats and a conserved C-terminal region. The locus 3p22.1 in the human chromosome harbors β-catenin gene. β-catenin shares 70% sequence identity to a protein encoded by Drosophila armadillo, a segment polarity gene. Both armadillo and β-catenin share considerable homology with plakoglobin, which has been proposed to be γ-catenin.

Specificity

Recognizes specifically mammalian and avian β-catenin. Does not cross-react with plakoglobin (γ-catenin).

Immunogen

recombinant chicken β-catenin.

Application

Monoclonal Anti-β-Catenin antibody produced in mouse has been used:
• immunoblotting
• immunohistochemistry
• immunolabeling
• immunofluorescence
• immunocytochemical staining

Physical form

The product is provided as ascites fluid with 0.1% sodium azide as a preservative.

Storage and Stability

For extended storage freeze in working aliquots. For continuous use, store at 2-8 °C for up to one month. Repeated freezing and thawing is not recommended. Storage in "frost-free" freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biochem/physiol Actions

β-catenin is a prime component of the wingless (Wnt) pathway and plays a key role in gene expression during development. The homology between β-catenin and armadillo raised the possibility that β-catenin has a developmental signaling role in vertebrates. β-catenin mediates the interaction of the E-cadherin-catenin complex with the epidermal growth factor (EGF) receptor. β-catenin and plakoglobin are substrates for tyrosine phosphorylation following EGF stimulation of cells. β-catenin also associates with the tumor suppressor protein adenomatous polyposis coli (APC). Monoclonal antibody reacting specifically with β-catenin is an essential tool in defining the interactions and distributions of β-catenin and its relationships with other catenins and cadherins in various cells and tissues.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 1
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable
Protocols & Articles

Articles

Antibody Basics

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Cancer Stem Cells: New Targets for Cancer Therapy

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Keywords: Cancer, Cell attachment, Cell culture, Cell proliferation, Gastrointestinal, Gene expression, Transcription, transformation

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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