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C7352 Sigma-Aldrich

L-Cysteine

from non-animal source, BioReagent, suitable for cell culture, ≥98%

Synonym: (R)-2-Amino-3-mercaptopropionic acid

  • CAS Number 52-90-4

  • Linear Formula HSCH2CH(NH2)CO2H

  • Molecular Weight 121.16

  •  Beilstein/REAXYS Number 1721408

  •  EC Number 200-158-2

  •  MDL number MFCD00064306

  •  eCl@ss 32160406

  •  PubChem Substance ID 24892959

  •  NACRES NA.75

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Description

General description

L-Cysteine is one of the sulfur containing proteinogenic amino acid incorporated into proteins as directed by the genetic code. The thiol-side chain participates in a variety of oxidation/reduction reactions within the cell. The side chain participates in the formation of β bonds that modulate the secondary and ternary structure of proteins. L-Cysteine is an essential precursor for inorganic sulfates, coenzyme A. Cysteine is an essential and limiting for the formation of glutathione, an important antioxidant, within the cell. Mammalian liver can synthesize L-cysteine and is a semi-essential amino acid. L-cysteine prevents oxidation of nerve cells, aids in maintaining the stability and rigidity of connective tissues. L-cysteine speeds up the healing process of the body and reduces inflammatory reactions. L-cysteine protects testis partially against acrylamide induced toxicity. L-cysteine can prevent DNA damage during cryopreservation and preserves semen quality.

L-Cysteine is a proteinogenic amino acid incorporated into proteins as directed by the genetic code. The thiol-side chain participates in a variety of oxidation/reduction reactions within the cell. The side chain participates in the formation of β bonds that modulate the secondary and ternary structure of proteins. Cysteine is essential and limiting for the formation of glutathione, an important antioxidant, within the cell.

Application

L-Cysteine has been used:
• as a media supplement in Trypticase-Yeast Extract-Iron-Serum (TYI-S-33) media for the culture of Giardia lamblia, to make enzyme solution in Dulbecco′s modified Eagles medium (DMEM) to establish hippocampus culture
• to test its protective effect on acrylamide induced toxicity in testis
• in the preparation of tris acetic acid extender solution for cryopreservation of semen
• as an additive to make papain digestion solution

Packaging

1 kg in poly bottle

10 mg in glass bottle

25, 100 g in poly bottle

Biochem/physiol Actions

NMDA glutamatergic receptor agonist.

Other Notes

L-Cysteine is used to supplement cell culture media. This product is a non-animal source amino acid useful for serum-free media formulations intended for biomanufacturing applications.

Tandem Mass Spectrometry data independently generated by Scripps Center for Metabolomics is available to view or download in PDF. C7352.pdf Tested metabolites are featured on Scripps Center for Metabolomics METLIN Metabolite Database. To learn more, visit sigma.com/metlin.

Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
1
RTECS 
HA1600000

Single- & multi-channel pipettes from BRAND

Pipette Controller from Brand
Protocols & Articles

Articles

Dietary Antioxidants

Antioxidants protect biological systems from oxidative damage produced by oxygen-containing free radicals and from redoxactive transition metal ions such as iron, copper, and cadmium.1 During the oxi...
Biofiles Volume 2 Article 2
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GC Analysis of Amino Acids (as TBDMS Derivatives) on SLB®-5ms (20 m x 0.18 mm I.D., 0.18 μm), Fast GC Analysis

From our library of Articles, Sigma-Aldrich presents GC Analysis of Amino Acids (as TBDMS Derivatives) on SLB®-5ms (20 m x 0.18 mm I.D., 0.18 μm), Fast GC Analysis
Keywords: Chromatography, Gas chromatography, Purification

HPLC Analysis of Cysteine Enantiomers on Astec® CHIROBIOTIC® T (Formic Acid Mobile Phase Additive)

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Cysteine Enantiomers on Astec® CHIROBIOTIC® T (Formic Acid Mobile Phase Additive)
Keywords: Chromatography, High performance liquid chromatography

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Peer-Reviewed Papers
15

References

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