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C7527 Sigma-Aldrich

Choline chloride

BioReagent, suitable for cell culture, suitable for insect cell culture, ≥98%

Synonym: (2-Hydroxyethyl)trimethylammonium chloride

  • CAS Number 67-48-1

  • Linear Formula (CH3)3N(Cl)CH2CH2OH

  • Molecular Weight 139.62

  •  Beilstein/REAXYS Number 3563126

  •  EC Number 200-655-4

  •  MDL number MFCD00011721

  •  PubChem Substance ID 24892968

  •  NACRES NA.75

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Properties

Related Categories Allium cepa (Onion), Allium sativum (Garlic), Aloe Vera, Artemisia vulgaris, Aspalathus linearis (Rooibos tea),
Quality Level   200
biological source   synthetic (organic)
product line   BioReagent
assay   ≥98%
form   crystals
mol wt   Mw 139.62 g/mol
application(s)   cell culture | insect: suitable
  cell culture | mammalian: suitable
  cell culture | plant: suitable
color   white
pH   5.0-6.5 (25 °C, 140 g/L)
mp   302-305 °C (dec.) (lit.)
solubility   H2O: 50 mg/mL
storage temp.   room temp
SMILES string   [Cl-].C[N+](C)(C)CCO
InChI   1S/C5H14NO.ClH/c1-6(2,3)4-5-7;/h7H,4-5H2,1-3H3;1H/q+1;/p-1
InChI key   SGMZJAMFUVOLNK-UHFFFAOYSA-M

Description

General description

Choline chloride is a quaternary ammonium salt and associates with hydrogen bond donors (HBD). It is essential for the vital physiological functions and is used as a nutritional additive. Choline is a source of methyl groups for methyl-transfer reactions.

Application

Choline chloride has been used:
• as a component of choline acetyltransferase (ChAT) buffer and for choline uptake in cultured human neuroblastoma (SK-N-SH) cells
• for choline standard curve generation for quantifying phospholipase D (PLD) activity
• as a component of slice solution for the dissection of entorhinal-hippocampal brain slices

Packaging

1 kg in poly drum

100, 500 g in poly bottle

Biochem/physiol Actions

The enzymatic activities of butyrylcholinesterase (BChE) and paraoxonase 1 (PON1), two serum enzymes synthesized by the liver and related with inflammation, were decreased in a sepsis animal model injected with LPS. Choline chloride administered intravenously at 20 mg/kg body weight prevents the LPS-mediated decreases in the activities of these two enzymes .

Substrates

Choline acetyltransferase substrate

Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 1
RTECS 
KH2975000
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Protocols & Articles

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Peer-Reviewed Papers
15

References

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