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C7611 Sigma-Aldrich

Monoclonal Anti-Caspase 12 antibody produced in rat

clone 14F7, purified immunoglobulin, buffered aqueous solution

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Apoptosis, Antibodies for Cell Biology, Antibodies to Caspases,
conjugate   unconjugated
clone   14F7, monoclonal
biological source   rat
application(s)   immunocytochemistry: suitable
  immunohistochemistry: suitable
  immunoprecipitation (IP): suitable
  microarray: suitable
  western blot: 1-2 μg/mL using whole cell extract of rat kidney cells NRK cells
species reactivity   rat, mouse
mol wt   antigen ~50 kDa
form   buffered aqueous solution
shipped in   dry ice
storage temp.   −20°C
antibody form   purified immunoglobulin
isotype   IgG2a
antibody product type   primary antibodies
UniProt accession no.   Q920D5
Gene Information   mouse ... Casp12(12364)
rat ... Casp12(156117)

Description

General description

Caspase 12 is a protein that belongs to a family of enzymes called caspases that cleave their substrates at C-terminal aspartic acid residues. The human caspase 12 gene is localized at a single locus within the caspase 1/ICE gene cluster on chromosome 11q22. The functionality is lost in humans and they cannot play a role in Alzheimer′s disease. Caspase-12 is detected in endoplasmic reticulum (ER), plays a role in ER-mediated apoptosis and inflammatory caspase activation pathway.

Specificity

Recognizes full length and possibly truncated p20 caspase 12.

Immunogen

recombinant mouse caspase 12 (residues 95-318).

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biochem/physiol Actions

Caspase 12 (CASP12) has a downregulatory function during infection and may play a role in protection against inflammatory disease. It is associated with rheumatoid arthritis in African-Americans. Caspase-12 inhibits caspase-1 activation and is associated with susceptibility factor for bacterial sepsis but does not influence the susceptibility to Candida sepsis.

Preparation Note

Purified from culture supernatant of hybridoma cells grown in a bioreactor.

Safety & Documentation

Safety Information

RIDADR 
UN 3082 9 / PGIII

Documents

Certificate of Analysis (COA)

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Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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