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C7714 Sigma

Monoclonal Anti-CD31 (PECAM-1) antibody produced in mouse

clone WM-59, purified immunoglobulin, buffered aqueous solution

Synonym: Anti-PECAM-1, Monoclonal Anti-CD31



Related Categories Alphabetical Index, Analytical Cytology, Antibodies, Antibodies for Cell Biology, Antibodies for Immune Cell Signaling and Blood,
species reactivity   human
application(s)   flow cytometry: 5 μL using 1 × 106 cells
clone   WM-59, monoclonal
antibody form   purified immunoglobulin
form   buffered aqueous solution
isotype   IgG1
mol wt   antigen mol wt 130-140 kDa
shipped in   wet ice
storage temp.   2-8°C
Gene Information   human ... PECAM1(5175)
biological source   mouse
conjugate   unconjugated



human cell line RC-2A, originally derived from myeloid leukemia cells.

Antigen Background

Human CD31 (PECAM-1) is expressed on platelets, endothelial cells, myeloid cells, B lymphocytes, and certain T lymphocyte subsets. CD31 (PECAM-1) functions in homophilic and heterophilic cell-cell adhesion and cell signaling activities.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.


Recognizes the CD31 (PECAM-1, endoCAM, gpIIa, hec7), human cell surface integral membrane glycoprotein. The antibody increases the rate of homotypic aggregation induced in U937 cells by TGF-β1. Its reactivity with platelets is enhanced following their washing.
5th Workshop: code No. P025

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WGK Germany  3
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Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

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P8590 Monoclonal Anti-CD31 (PECAM-1) antibody produced in mouse, clone WM-59, purified immunoglobulin, buffered aqueous solution

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