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  • C7738 - Anti-β-Catenin−Cy3 antibody, Mouse monoclonal

C7738 Sigma-Aldrich

Anti-β-Catenin−Cy3 antibody, Mouse monoclonal

clone 15B8, purified from hybridoma cell culture

Synonym: Monoclonal Anti-β-Catenin



Related Categories AA - CA, Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Epigenetics and Nuclear Signaling,
conjugate   CY3 conjugate
clone   15B8, monoclonal
biological source   mouse
application(s)   direct immunofluorescence: 1:100 using methanol/acetone-fixed bovine MDBK/MCF-7 Cell Line
species reactivity   human, bovine, chicken, canine
mol wt   antigen 94 kDa
form   buffered aqueous solution
shipped in   wet ice
storage temp.   2-8°C
antibody form   purified immunoglobulin
isotype   IgG1
antibody product type   primary antibodies
concentration   ~1 mg/mL
UniProt accession no.   P35222
Gene Information   human ... CTNNB1(1499)


General description

Anti-β-Catenin-Cy3 antibody, Mouse monoclonal (mouse IgG1 isotype) is derived from the 15B8 hybridoma, produced by fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with recombinant chicken catenin. β-catenin is 92 kDa. β-catenin protein is composed of a series of protein-protein interaction motifs that allow it to function as a scaffold. The N-terminus domain contains the binding site for β-catenin as well as phosphorylation sites recognized by Glycogen synthase kinase 3 beta (GSK3β) and the C-terminus contains the transcriptional activation domain and binding site for Teashirt.

The gene β-catenin (CTNNB1) is mapped to human chromosome 3p21. The protein localizes to the cytoplasm and the nucleus. β-catenin is also present at the centrosomes.


Recognizes specifically mammalian and avian β-catenin. Does not cross-react with plakoglobin (γ-catenin).


recombinant chicken β-catenin.


Anti-β-Catenin-Cy3 antibody has been used in
• direct immunofluorescence
• western blotting
• staining
• immunohistochemistry

Suitable for direct immunofluorescence at a dilution of 1:100 using methanol/acetone-fixed bovine MDBK cells. The antiobody has been used for western blot analysis in mouse embryonic stem cells.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.


Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Legal Information

Cy is distributed under license from Amersham Biosciences Limited.


200 μL in glass insert

Biochem/physiol Actions

Catenin (cadherin-associated protein) β 1 also called β-catenin is encoded by the CTNNB1 gene in humans. It is a dual function protein that regulates the coordination of cell-cell adhesion and gene transcription. It is useful for differential diagnosis of osteoblastoma (OB) and osteosarcoma (OS). β-catenin signaling pathway may contribute to cholangiocarcinoma (CCA) cell proliferation. It may serve as a prognostic marker for CCA progression and provide a potential target for CCA therapy. Mutations in β-catenin are associated with ovarian carcinoma. In addition, mutations in β-catenin are linked to intellectual disability. NEK2 (Never in mitosis A-related kinase 2) binds and phosphorylates β-catenin, thereby regulating centrosome seperation. Focal adhesion protein, Kindlin-2, enhances WNT signaling by forming a transcriptional complex with β-catenin.

Safety & Documentation

Safety Information

NONH for all modes of transport
WGK Germany 
Protocols & Articles


Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

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