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C7762 Sigma-Aldrich

α-Chymotrypsin from bovine pancreas

Type I-S, essentially salt-free, lyophilized powder

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Properties

Related Categories 3.4.x.x Peptidases, 3.x.x.x Hydrolases, Application Index, Biochemicals and Reagents, Chymotrypsin, alpha-,
type   Type I-S
form   essentially salt-free, lyophilized powder
activity   ≥40 units/mg protein
mol wt   25 kDa
purified by   3× crystallization
solubility   1 mM HCl: soluble 2.0 mg/mL, clear
storage temp.   −20°C
Gene Information   cow ... CTRB1(618826)

Description

Application

α-Chymotrypsin from bovine pancreas has been used in a study to investigate protein extraction by Winsor-III microemulsion systems. α-Chymotrypsin from bovine pancreas has also been used in a study to investigate a new specific fullerene-based fluorescent probe for trypsin.

The product has been used to investigate the inhibitory effect of several ether oligomers against the enzyme. It has also been used to cleave pro-phenoloxidase in order to estimate total phenoloxidase in haemolymph. Furthermore, the enzyme has been used as a positive control in chymotrypsin assays using salivary gland and anterior midgut extracts of Deraeocoris nigritulus.

Packaging

5, 25, 100 mg in glass bottle

Biochem/physiol Actions

α-Chymotrypsin is a serine peptidase and has 241 amino acid residues contained in three polypeptide chains (A chain-13 residues, B chain-131 residues, and C chain-97 residues) linked by disulfide bridges. Molecular weight of this enzyme is found to be 25 kDa. The pI is 8.75. It selectively hydrolyzes peptide bonds on the C-terminal side of tyrosine, phenylalanine, tryptophan, and leucine. Ca2+ activates and stabilizes the enzyme. The enzyme is inhibited by diisopropyl fluorophosphate (DFP), phenylmethanesulfonyl fluoride (PMSF), N-p-tosyl-L-phenylalanine chloromethyl ketone (TPCK), chymostatin, aprotinin, α1-antitrypsin, and α2-macroglobulin, as well as 10 mM of Cu2+ and Hg2+.

A serine protease that hydrolyzes peptide bonds with aromatic or large hydrophobic side chains (Tyr, Trp, Phe, Met, Leu) on the carboxyl end of the bond.

Unit Definition

One unit will hydrolyze 1.0 μmole of BTEE per min at pH 7.8 at 25 °C.

Preparation Note

Prepared free of autolysis products and low molecular weight contaminants.

The powder may be reconstituted in 1 mM HCl at 2 mg/mL concentration to form a clear solution.

Analysis Note

Minimum 85% protein

Protein determined by E1%/280

Other Notes

View more information on chymotrypsin at www.sigma-aldrich.com/enzymeexplorer

Safety & Documentation

Safety Information

Symbol 
Signal word 
Danger
Hazard statements 
Precautionary statements 
Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
1
RTECS 
GC3050000

Documents

Certificate of Analysis

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Certificate of Origin

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Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
How do I find price and availability?
There are several ways to find pricing and availability for our products.Once you log onto our website, you will find the price and availability displayed on the product detail page.You can contact any of our Customer Sales and Service offices to receive a quote. USA customers: 1-800-325-3010 orview local office numbers.
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
How should I prepare a solution of α-Chymotrypsin, Product C7762?
It is recommended to reconstitute product C7762 in 1 mM hydrochloric acid containing 2 mM calcium chloride. The calcium functions as both a stabilizer (and possibly an activator) of the enzyme.
How can solutions of α-Chymotrypsin, Product C7762, be stored?
Stock solutions prepared in 1 mM HCl, containing 2 mM calcium chloride can be stored at -20 °C for about one week.
What is the pH optimum for the enzyme, α-Chymotrypsin, Product C7762?
The pH optimum is between pH 7.5-8.5.
What is the molecular weight of alpha-chymotrypsin from bovine pancreas (Product No. C7762)?
The molecular weight of alpha chymotrypsin, reported in the literature, is approximately 25 kDa.This information and the basic structure description of the enzyme can be found on our product information sheet (under Documents, above).
My question is not addressed here, how can I contact Technical Service for assistance?
Ask a Scientist here.
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Protocols & Articles

Articles

HPLC Analysis of Proteins on TSKgel® Butyl-NPR

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Proteins on TSKgel® Butyl-NPR
Keywords: Chromatography, High performance liquid chromatography

HPLC Analysis of Proteins on TSKgel® Phenyl-5PW

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Proteins on TSKgel® Phenyl-5PW
Keywords: Chromatography, High performance liquid chromatography

Protocols

Procedure for Enzymatic Assay of α-Chymotrypsin (EC 3.4.21.1)

This procedure may be used for the determination of α‑Chymotrypsin activity using N-Benzoyl-L-tyrosine ethyl ester (BTEE) as the substrate. It is not to be used to assay α‑Chymotrypsin agarose (Catal...
Keywords: Extinction coefficient

Related Content

Enzymes & Proteins

Application Index | Enzyme Index | Substrate Index | Inhibitor Index | Cofactor Index | Lectin Index
Keywords: Cell signaling, Diagnostic, Drug discovery, Metabolomics, Molecular biology

Peer-Reviewed Papers
15

References

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