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C7930 Sigma-Aldrich

Anti-CD3, T Cell antibody produced in rabbit

whole antiserum

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Properties

Related Categories Alphabetical Index, Analytical Cytology, Antibodies, Antibodies against Proteins/Bioactives/Markers/Receptors for Stem Cell Biology, Antibodies for Cell Biology,
conjugate   unconjugated
clone   polyclonal
biological source   rabbit
application(s)   immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:200 using enzyme pre-digested, formalin-fixed, paraffin-embedded human tonsil sections
  western blot: 1:200 using mouse thymus extract
species reactivity   pig, canine, human, mouse, feline, bovine, sheep, chicken, Psammomys (sand rat), rat
mol wt   antigen 20 kDa (bovine and swine)
shipped in   dry ice
storage temp.   −20°C
antibody form   whole antiserum
antibody product type   primary antibodies
contains   15 mM sodium azide
UniProt accession no.   P07766
Gene Information   human ... CD3E(916)
mouse ... Cd3e(12501)
rat ... Cd3e(315609)

Description

General description

CD3 is a human cell surface glycoprotein complex involved in signal transduction following binding of antigen receptor. The CD3 antigen is expressed during thymopoiesis and on mature T cells in the periphery. It is also present in the majority of T-CLL and T-lymphomas and in approximately 70% of T-ALL. Cytoplasmic CD3 was recently demonstrated in 95% of thymocytes and in all T-ALL including those in which surface membrane CD3 was absent. Cerebellar Purkinje cells were also reported to express the antigen. B lymphocytes, monocytes, granulocytes and NK cells do not express it.

Rabbit polyclonal anti-CD3, T cell antibody is reactive with T cell areas in lymphoid tissues derived from various species: human, bovine, porcine, sheep, cat, dog, rat, mouse, Psammomys (sand rat), and chicken. The molecular weight of the polypeptide recognized by the antibody in bovine and swine extracts is 20 kDa. The antibody may contain residual antibodies to thyroglobulin and may exhibit minimal background staining of epithelial and muscle tissues.
Anti-Human T Cell CD3 Peptide was first described by Mason et al. The antibody reacts with the proline-rich peptide sequence comprising 13 amino acids in the cytoplasmic domain of the CD3-ε chain. It detects normal, reactive and most neoplastic human T cells in routinely processed enzyme pre-digested, formalin-fixed, paraffin-embedded tissue sections. The antibody also reacts with human tissues fixed with Bouin′s solution and embedded in paraffin. Frozen sections and smears are also suitable for staining with this antibody. The antibody reacts with T cell areas in lymphoid tissues derived from various species.

Specificity

The antibody reacts with the proline-rich peptide sequence comprising 13 amino acids in the cytoplasmic domain of the CD3-ε chain. It detects normal, reactive and most neoplastic human T cells in routinely processed enzyme pre-digested, formalin-fixed, paraffin-embedded tissue sections. The antibody also reacts with human tissues fixed with Bouin′s solution and embedded in paraffin. Frozen sections and smears are also suitable for staining with this antibody. The antibody reacts with T cell areas in lymphoid tissues derived from various species.

Immunogen

synthetic human CD3 peptide (residues 156-168 of the CD3-ε chain) coupled to porcine thyroglobulin. Coupling was performed by a modification of the reported method.

Application

Rabbit polyclonal anti-CD3, T cell antibody may be used for identification of T lymphocytes in peripheral blood and tissues; detection and classification of T-ALL, lymphomas and immunodeficiencies and studies on detection and purification of CD3 from lymphocytes of different species. The antibody may be used in immunoblotting and to stain T cell areas in immunohistology.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
2

Documents

Certificate of Analysis

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Protocols & Articles

Articles

Antibody Basics

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Protocols

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Peer-Reviewed Papers
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References

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