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C7999 Sigma-Aldrich

Collagen human

Bornstein and Traub Type I, recombinant, expressed in Nicotiana tabacum (tobacco)

Synonym: collage

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Description

General description

Collagen is classified into a number of structurally and genetically distinct types. We use the nomenclature proposed by Bornstein and Traub. Do not confuse Sigma type designations with recognized collagen classification types.

Application

This recombinant type I human collagen is suitable for cell culture applications, including surface coating and the fabrication of scaffolds and 3-Dimensional matrices. This collagen is prepared from bioengineered plants and is free of human-derived components such as as cytokines, hormones, and growth factors.

Preparation Note

Resuspend in 10 mM HCl to the desired concentration. Good solubility expected at a concentration between 0.3% and 10%.
Coating of tissue culture dishes: Use 3 to 10 microgram per 1 cm2 dish area. For each cell type and plate type an optimization may be required. Dilute in 10 mM HCl or PBS. Apply the diluted collagen to a tissue culture plate and allow to dry overnight in a laminar flow hood with the plate lid open. Aspirate gently the remaining liquid from the coated wells. Wash twice gently with PBS.
To prepare a collagen gel the fibrillogenesis buffer contains 162 mM sodium phosphate dibasic (Na2HPO4) adjusted to pH 11.2 with 10 N NaOH, filter sterilized. Mix 9 volumes of Collage at a concentration of 0.3%-1% with 1 volume of fibrillogenesis buffer. Mix well and incubate for 4 to 16 hours at 25°C to 27 °C. Higher concentration of collagen solution might need optimization. Thiis preparation will not spontaneously form a gel in PBS or cell culture medium.
Preparation of collagen sponge: concentrate gel to 10 mg/ml - 100 mg/ml by centrifugation, freeze dry. Crosslink by common methods found in the scientific literature such as 1-Ethyl-3-[3-dimethylaminopropyl]carbodiimide hydrochloride (EDC), Glutaraldehyde or thermal dehydration.
Other forms: membranes, sheets, fibers can be prepared by methods commonly used with other types of collagen.

Reconstitution

Soluble in 10 mM HCl at ≤ 3.5 mg/mL. Solutions should be stored at 2-8 degrees C.

Legal Information

Collage is a trademark of CollPlant Ltd.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
1

Documents

Certificate of Analysis

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Cell Culture Guide
Protocols & Articles

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References

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