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  • C8102 - Anti-CIDE-B, C-Terminal antibody produced in rabbit

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C8102 Sigma-Aldrich

Anti-CIDE-B, C-Terminal antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

Synonym: Anti-Cell Death-Inducing DFF-like Effector B

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Apoptosis, Antibodies for Cell Biology, Antibodies to DNA Fragmentation,
conjugate   unconjugated
clone   polyclonal
biological source   rabbit
application(s)   western blot: 1 μg/mL using mouse heart tissue lysate
species reactivity   mouse
mol wt   antigen 25 kDa
form   buffered aqueous solution
shipped in   dry ice
storage temp.   −20°C
antibody form   affinity isolated antibody
antibody product type   primary antibodies
UniProt accession no.   O70303
Gene Information   mouse ... Cideb(12684)

Description

General description

CIDE-B is consists of a twisted five-stranded β-sheet and two α helices arranged in an α⁄β roll fold. It is expressed mainly in liver and at lower levels in spleen, kidney, peripheral blood lymphocytes and bone marrow.

Immunogen

synthetic peptide corresponding to amino acids 204-219 of the C-terminal of mouse CIDE-B.

Application

Anti-CIDE-B, C-Terminal antibody is suitable for western blot at a dilution of 1 μg/mL by using mouse heart tissue lysate.

Physical form

Solution in phosphate buffered saline containing 0.02% sodium azide.

Biochem/physiol Actions

Apoptosis is related to many diseases and induced by a family of death receptors and their ligands. CIDE-B induces DNA fragmentation and activates apoptosis in mammalian cells. The apoptotic activity of CIDE-B is inhibited by DFF40/CAD (DNA Fragmentation Factor 40/caspase-activated DNase).

Safety & Documentation

Safety Information

WGK Germany 
3
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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