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C8118 Sigma-Aldrich

Chymase human

recombinant, expressed in Pichia pastoris

Synonym: Mast cell protease I

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Properties

Related Categories 3.4.x.x Peptidases, 3.x.x.x Hydrolases, Application Index, Biochemicals and Reagents, Cell Biology,
Quality Level   PREMIUM
recombinant   expressed in Pichia pastoris
activity   ≥40 units/mg protein
concentration   125-400 μg/mL
Featured Industry   Diagnostic Assay Manufacturing
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... CMA1(1215)

Description

Application

Human chymase has been used in a study to assess the effects of Panax notoginseng flower extract on the TGF-β/Smad signal transduction pathway in heart remodeling. Human chymase has also been used in a study to investigate the blood glucose level and survival in streptozotocin-treated human chymase transgenic mice.

Chymase has been implicated in generation of angiotensin II and cleavage of big endothelin. Studies indicate it may be involved in vascular proliferation, myocardial infarction and dermatitis.

Biochem/physiol Actions

The enzyme rapidly converts angiotensin I to angiotensin II. Optimum pH for the enzyme activity is between 7.5 and 9.0. Enzyme activity is inhibited by soybean trypsin inhibitor, phenylmethylsulfonyl fluoride and chymostatin.

Physical properties

Chymase is a cathepsin G-like, S1 serine proteinase found primarily in mast cells. It has a molecular mass of ~30 kDa, however its apparent molecular mass on SDS-PAGE is around 37 kDa.

Unit Definition

One unit hydrolyzes one micromole of N-benzoyl-L-tyrosine ethyl ester (BTEE) per minute at pH 7.8 and 25 °C. The assay buffer used to determine the enzyme activity contains 27 mM Tris-HCl, pH 7.8, with 150 mM NaCl and 0.43 mM BTEE.

Physical form

Supplied as a solution in 20 mM Tris, 0.8 M NaCl and 25% glycerol, pH 7.6

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
1

Documents

Certificate of Analysis

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Milli-Q® Water Purification Solutions
Protocols & Articles

Related Content

Enzymes & Proteins

Application Index | Enzyme Index | Substrate Index | Inhibitor Index | Cofactor Index | Lectin Index
Keywords: Cell signaling, Diagnostic, Drug discovery, Metabolomics, Molecular biology

Peer-Reviewed Papers
15

References

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CS1140 Chymase Activity Assay Kit, sufficient for 150 assays (100 μL), sufficient for 30 assays (1 mL)

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