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C8302 Sigma-Aldrich

4-Chloro-1-naphthol solution

For HRP detection on Western Blots

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Properties

Related Categories Biochemicals, Biochemicals and Reagents, Colorimetric, Detection, Enzyme Substrates,
Quality Level   200
grade   for molecular biology
form   liquid
storage temp.   2-8°C
SMILES string   Oc1ccc(Cl)c2ccccc12
InChI   1S/C10H7ClO/c11-9-5-6-10(12)8-4-2-1-3-7(8)9/h1-6,12H
InChI key   LVSPDZAGCBEQAV-UHFFFAOYSA-N

Description

General description

4-Chloro-1-naphthol is a substrate for HRP in a reaction that results in a colored precipitate. It is commonly used for colorimetric detection and visualization of proteins in western blotting.

Application

Suitable for visualizing protein-peroxidase conjugates in western blotting.

Packaging

100 mL in poly bottle

Components

This product contains 0.48 mM 4-chloro-1-naphthol, 50 mM Tris-HCl, 0.2 M NaCl and 17% methanol.

Other Notes

0.48 mM 4-chloro-1-naphthol, 50 mM Tris-HCl, 0.2 M NaCl and 17% methanol.

Principle

Enzymes such as HRP convert 4-Chloro-1-naphthol to a purple-colored precipitate on the blot that is readily visible to the eye.

Safety & Documentation

Safety Information

Symbol 
Signal word 
Danger
Hazard statements 
Target organs 
Eyes
RIDADR 
NA 1993 / PGIII
WGK Germany 
WGK 2
Flash Point(F) 
163.9 °F - closed cup
Flash Point(C) 
73.3 °C - closed cup

Documents

Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Protocols & Articles

Articles

Colorimetric Alkaline Phosphatase and Peroxidase Substrate Detection Systems

Nitroblue Tetrazolium (NBT) is used with the alkaline phosphatase substrate 5-Bromo- 4-Chloro-3-Indolyl Phosphate (BCIP) in western blotting and immunohistological staining procedures. These substrat...
BioFiles 2008, 3.4, 6.
Keywords: Enzyme-linked immunosorbent assay, Immunohistochemistry, Western blot

Intro to Detection Methods Used in Molecular Biology

Different detection methods are used to visualize the nucleic acids and specific proteins transferred to membrane support. Southern and northern blotting involves the use of radioactive probes and th...
Keywords: Catalytic combustion detector, Detection methods, Northern blot, Nucleic acid hybridization, Purification, Size-exclusion chromatography, Western blot

Protocols

4-Chloro-1-Naphthol Solution Protocol

Solution contains 0.48 mM 4-Chloro-1-Naphthol, 50 mM Tris-HCl and 0.2 M NaCl in 17% methanol. This is a substrate solution designed for visualizing horseradish peroxidase conjugates in Western Blotti...
Keywords: Western blot

Peer-Reviewed Papers
15

References

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