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C8482 Sigma-Aldrich

Protein Kinase A catalytic subunit human

>90% (SDS-PAGE), recombinant, expressed in E. coli, buffered aqueous glycerol solution

Synonym: Cyclic AMP-dependent protein kinase catalytic subunit, histidine-tagged, recombinant human

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Properties

Related Categories Active Kinases, Application Index, Biochemicals and Reagents, Cell Biology, Cell Signaling Enzymes,
Quality Level   200
recombinant   expressed in E. coli
assay   >90% (SDS-PAGE)
form   buffered aqueous glycerol solution
specific activity   >1000 units/mg protein
mol wt   43.5 kDa
shipped in   dry ice
storage temp.   −70°C
Gene Information   human ... PRKACA(5566)

Description

Biochem/physiol Actions

Ubiquitous serine-threonine kinase that phosphorylates a broad spectrum of substrates, and regulates many cellular processes. The catalytic subunit is released following binding of cyclic AMP to the regulatory subunits of the PKA holoenzyme. The free catalytic subunit has intrinsic activity and does not require added cyclic AMP.

Physical form

Solution in 30 mM potassium phosphate buffer, pH 7.4, containing 50% glycerol, 150 mM KCl, 1 mM EDTA, and 1 mM DTT.

Analysis Note

One unit will transfer 1 nanomole of phosphate from 32P-ATP to kemptide substrate per minute at pH 7.4 at 30 °C (measured by a radioactive filter-binding assay).
Km (ATP) = 25 μM at 30 °C, pH 7.4. Km (kemptide) = 42 μM (33 μg/ml) at 30 °C, pH 7.4.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 1
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Protocols & Articles
Peer-Reviewed Papers
15

References

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