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C8571 Sigma-Aldrich

Cathepsin B from human liver

buffered aqueous solution, ≥1,500 units/mg protein (E1%/280)

Synonym: Cathepsin B1

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Description

General description

Cathepsin B gene is mapped to human chromosome 8p23.1. It is a lysosomal cysteine with 252 amino acids distributed in two chains and is homologous to rat liver sequence. It has disc shaped structure with two domains namely the left-hand ′L′ domain at the amino terminal and right hand ′R′ domain at the carboxyl-terminal.

Application

Cathepsin B from human liver has been used:
• as a positive control in the screening Cathepsin B inhibitor assay
• in wall extension (expansin activity) assay
• in microinjection human foreskin fibroblasts to test its effect on apoptosis induction

Biochem/physiol Actions

Cathepsin B from human liver is highly expressed in the esophageal adenocarcinoma (EAC). It reduces hepatic inflammation and fibrogenesis and may serve as a potential candidate for treating liver diseases.

Cathepsin B has been found to cleave procaspase 1 and procaspase 11 and to induce apoptosis in digitonin-permeabilized cells. Translocation of cathepsin B from the cytoplasm to the nucleus contributes to bile salt induced apoptosis of rat hepatocytes. Levels of cathepsin B in PC12 cells significantly decrease 12 to 24 hours after apoptosis is induced.

Packaging

Package size based on protein content

Unit Definition

One unit will liberate 1 nanomole of 7-amino-4-methylcoumarin from Z-Arg-Arg 7-amido-4-methylcoumarin per min at pH 6.0 at 40 °C.

Physical form

Solution in 50 mM sodium acetate, pH 5.0, with 1 mM EDTA.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

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Certificate of Origin

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Milli-Q® Water Purification Solutions
Protocols & Articles

Protocols

Enzymatic Assay of Cathepsin B

This procedure applies to all products that have a specification for Cathepsin B activity, such as Sigma-Aldrich Product Numbers C0150 and C8571, determined by the liberation of 7-amino-4-methylcouma...
Keywords: Enzyme activity, Enzymology

Related Content

Enzymes & Proteins

Application Index | Enzyme Index | Substrate Index | Inhibitor Index | Cofactor Index | Lectin Index
Keywords: Cell signaling, Diagnostic, Drug discovery, Metabolomics, Molecular biology

Peer-Reviewed Papers
15

References

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related product

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C6243 Anti-Cathepsin B antibody, Mouse monoclonal, clone CB59-4B11, purified from hybridoma cell culture
92210 Timestrip Plus 0 °C
06693 Timestrip Plus -20 °C
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substrate

Product #

Description

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H2508 Hippuryl-Arg, carboxypeptidase substrate
C8536 Z-Ala-Arg-Arg 4-methoxy-β-naphthylamide acetate salt
S6510 N-Succinyl-Leu-Leu-Val-Tyr-7-Amido-4-Methylcoumarin, ≥90% (HPLC)
C5429 Z-Arg-Arg-7-amido-4-methylcoumarin hydrochloride

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