• USA Home
  • C8919 - Collagen from calf skin

EMAIL THIS PAGE TO A FRIEND
C8919 Sigma-Aldrich

Collagen from calf skin

Bornstein and Traub Type I, (0.1% solution in 0.1 M acetic acid), aseptically processed, BioReagent, suitable for cell culture

Purchase

Description

Components

All collagen molecules are composed of three polypeptide chains arranged in a triple helical conformation, with a primary structure that is mostly a repeating motif with glycine in every third position and proline or 4-hydroxyproline frequently preceding the glycine residue. Type I collagen differs from other collagens by its low lysine hydroxylation and low carbohydrate composition.

Application

This product is intended to produce thin layer coatings on tissue culture plates to facilitate attachment of anchorage-dependent cells, recommended for use at 6-10 μg/cm2. It is NOT intended for production of 3-D gels. Type I collagen is often used in cell culture as an attachment substratum with myoblasts, spinal ganglia, hepatocytes, embryonic lung, heart explants, fibroblasts, endothelial cells, and islet cells have all been cultured successfully on films or gels of type I collagen. Collagen type I may also be used in research of Idiopathic pulmonary fibrosis (IPF), studies on the effect of ER stress IPF on lung fibroblasts. Collagen in acidic solution can produce three dimensional scaffolding with use in bioengineering and cell culture applications.

Collagen from calf skin has been used:
• for pre-coting glass slides for immunofluorescence studies
• as a cell adhesion factor and modification of poly(vinylidene fluoride-trifluoroethylene) (P(VDF-TrFE)) films for neuron culture
• for coating culture dishes for murine embryonic fibroblasts culture

Other Notes

Collagen is classified into a number of structurally and genetically distinct types. We use the nomenclature proposed by Bornstein and Traub. Do not confuse Sigma type designations with recognized collagen classification types.

Preparation Note

This product is a 0.1% (1 mg/mL) solution of calf skin collagen 0.1 M acetic acid. Please note the product information sheet for details on procedure prior to introduction to cells and media.

Biochem/physiol Actions

Collagen type I on heat denaturation results in disruption of triple helix to a randomly coil. Mutations in the collagen gene are implicated in a variety of cattle diseases. It has applications in food and cosmetics. Collagen is used as a biomaterial and as a tissue scaffold in tissue engineering. Collagen type I fiber assembly is very crucial for physiological processes and cellular signaling.

General description

Collagen type I is a component of skin, bone, tendon, and other fibrous connective tissues. It consists of repeating triplet amino acids glycine, proline and hydroxyproline. Collagen is a left handed helix with three polypeptide chains. It is a component of extracellular matrix and as close to 28 types are present in bovine.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
nwg

Frequently Asked Questions

How can I sterilize collagen solutions?
We recommend transferring the collagen solution to a glass bottle with a screw cap and carefully layering chloroform at the bottom. The amount of chloroform to use should be ~10% of the volume of collagen solution. DO NOT SHAKE OR STIR. Allow to stand overnight at 28 °C. Aseptically remove the top layer containing the collagen solution to a new bottle. We do not recommend sterilizing the collagen solution by membrane filtration. We have found substantial protein loss by this method.
Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
CanProduct C8919, Collagen solution, be used to make 3-D collagen gels?
This product is NOT intended to produce 3-D (thick) gels, since the solution will not gel. This may be due to gamma-irradiation of the powder during the production process.We recommend using Product No C4243, which is tested and found to be suitable for 3-D gel formation.
Why does Product C8919, Collagen solution, look milky or contain particulates?
C8919, Collagen Solution from calf skin, is prepared by allowing irradiated sterile collagen strands to mix with sterile filtered 0.1N acetic acid over several hours. The collagen dissolves to an opalescent solution that is aseptically dispensed while stirring. No further filtration is necessary. Due to cross-linkage and sequence variability, as well as structural changes that can occur during isolation and purification, Collagen Type I (Bornstein and Traub) is a heterogeneous mixture rather than a pure compound. This variability, along with slight differences in the raw material, lends itself to shifts in the opacity of the solution from lot to lot. This product is tested for sterility prior to release for sale to our customers. A milky or particulate appearance is not indicative of microbial contamination and does not affect product use. .
How can I use Product C8919, Collagen solution, to coat my cultureware?
A protocol for coating cultureware can be found on the product information sheet (under Documents, above).
How long can plates coated with Product C8919, Collagen solution, be stored?
We make no recommendations about storage of plates after coating.Since factors such as humidity may impact on longevity of coated plates, customers should make their own determination as to whether plates can be stored and under which conditions.We used to offer SigmaScreen collagen coated plates as Product No. S3315. For optimal performance of that product, we recommend that the unopened product should be stored in a dry place at 2-8°C. We found that the S3315 could be stored at room temperature for up to three months, and that it should never be exposed to temperatures above 50°C.
Product C8919, Collagen solution, gelled while refrigerated. Can it still be used?
This can occur but does not harm the product. When warmed to room temperature, the product will liquify. Please make sure it is well mixed prior to dilution for coating cultureware.
How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
How do I find price and availability?
There are several ways to find pricing and availability for our products.Once you log onto our website, you will find the price and availability displayed on the product detail page.You can contact any of our Customer Sales and Service offices to receive a quote. USA customers: 1-800-325-3010 orview local office numbers.
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
My question is not addressed here, how can I contact Technical Service for assistance?
Ask a Scientist here.
Show more questions

Request a Serum Sample

Cell Culture Guide
Protocols & Articles

Articles

Attachment Factors for 3-Dimensional Cell Culture

Three-dimensional (3D) extracellular matrix-based cell culture systems are being used as research tools for understanding normal and disease systems, and for drug screening in vitro. The extracellula...
Efrat Manaster
BioFiles 2008, 3.8, 16.
Keywords: Adhesion, Angiogenesis, Biomaterials, Cancer, Cell biology, Cell culture, Clinical, Gene expression, Genetic, Genetics, Genomics, Growth factors, Hormones, Molecular biology, Morphogenesis

Cancer Stem Cells: New Targets for Cancer Therapy

Interested in receiving more content like this? Join over 8,000 of your peers who have already signed up for the Cell Informer newsletter.
Keywords: Cancer, Cell attachment, Cell culture, Cell proliferation, Gastrointestinal, Gene expression, Transcription, transformation

Collagen Attachment Protocols, Solubility, and Stability

A1) We recommend transferring the collagen solution to a glass bottle with a screw cap and carefully layering chloroform at the bottom. The amount of chloroform to use should be ~10% of the volume of...
George Sitterley
BioFiles 2008, 3.8, 5.
Keywords: Centrifugation, Dialysis, Filtration, Purification, Sterilizations

Collagen Usage Recommendations

Collagen is one of the most abundant proteins in the connective tissues and internal organs of mammals. It provides the tensile strength of the extracellular matrix (ECM). It is classified into a num...
George Sitterley
BioFiles 2008, 3.8, 4.
Keywords: Cell culture, Morphogenesis

Extracellular Matrix Proteins and tools for cell culture optimization

Animal cells and tissue culture techniques are constantly improved to optimize in vitro cell culture conditions. Extracellular Matrix (ECM) proteins coating, chemical or physical modification of the ...
Keywords: Adhesion, Angiogenesis, Apoptosis, Asymmetric synthesis, Cancer, Cell attachment, Cell culture, Cell proliferation, Coagulation, Endocrinology, Growth factors, Hormones

What is the Extracellular Matrix?

The extracellular matrix (ECM) is secreted by cells and surrounds them in tissues. It has long been understood to be the structural support for cells since its characteristics set the characteristics...
Keywords: Adhesion, Cell culture, Cellular processes, Epimerizations, Growth factors, Immobilization, Sulfations

Related Content

Cell Culture Troubleshooting Guide

Cell culture has become one of the most fundamental techniques for modeling biological systems, and is of increasing importance in the biotechnology and pharmaceutical sectors as well as an essential...
Keywords: Antibiotics, Apoptosis, Cell attachment, Cell culture, Cryopreservation, Filtration, Growth factors, Pharmaceutical, Phase transitions

Peer-Reviewed Papers
15

References

Related Products

related product

Product #

Description

Add to Cart

08168 Timestrip Plus 8 °C

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?