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C9080 Sigma-Aldrich

Monoclonal Anti-Vimentin−Cy3 antibody produced in mouse

clone V9, purified immunoglobulin, buffered aqueous solution

Synonym: Monoclonal Anti-Vimentin

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies against Proteins/Bioactives/Markers/Receptors for Stem Cell Biology, Antibodies for Alzheimer′s Disease, Antibodies for Cardiac Stem Cells,
conjugate   CY3 conjugate
clone   V9, monoclonal
biological source   mouse
application(s)   direct immunofluorescence: 1:200 using cultured cells
  immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50 using human tonsil
  immunohistochemistry (frozen sections): suitable using human tonsil
species reactivity   chicken, rat, bovine, feline, hamster, gerbil, horse, canine, rabbit, monkey, pig, human
mol wt   antigen mol wt ~58 kDa
form   buffered aqueous solution
shipped in   wet ice
storage temp.   2-8°C
antibody form   purified immunoglobulin
isotype   IgG1
antibody product type   primary antibodies
UniProt accession no.   P08670
Gene Information   human ... VIM(7431)
rat ... Vim(81818)

Description

General description

Vimentin is one of the five major groups of intermediate filaments with a molecular mass of 57 kDa. Intermediate filaments (IFs) with characteristic 10 nm diameter are a distinct class of molecularly heterogenous cytoskeletal filaments defined by ultrastructural, immunological and biochemical criteria. IFs differ significantly from the other cytoskeletal elements of the cell, namely microtubules and microfilaments and are components of most eukaryotic cells.

Specificity

The antibody localizes vimentin in fibroblasts, endothelial cells, lymphoid tissue and melanocytes in immunohistochemical staining. It also stains vimentin in sarcomas, lymphomas, and melanomas.

Immunogen

pig eye lens vimentin.

Application

Cy3 Monoclonal Anti-Vimentin may be used:
• to study the expression of vimentin in cultured cells
• for immunohistochemistry and immunocytochemistry
• for double labeling experiments with fluorescein tagged antibodies
• in testing the adequacy of tissue fixation and for monitoring the recovery from over fixation achieved by antigen retrieval methodologies
• for western blotting

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide

Storage and Stability

Store at 2-8 °C. Protect from prolonged exposure to light. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Legal Information

Cy is distributed under license from Amersham Biosciences Limited.

Biochem/physiol Actions

Vimentin was found to be important for stabilizing the architecture of the cytoplasm. It was found, that activated macrophages secrete vimentin into the extracellular space in vitro.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
nwg

Milli-Q® Water Purification Solutions
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

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Peer-Reviewed Papers
15

References

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