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C9179 Sigma-Aldrich

Monoclonal Anti-Cytokeratin Peptide 17 antibody produced in mouse

clone CK-E3, ascites fluid

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies to Cell and Organelle Proteins, Antibodies to Cytokeratins,
conjugate   unconjugated
clone   CK-E3, monoclonal
biological source   mouse
application(s)   indirect immunofluorescence: 1:400 using acetone-fixed frozen sections of human salivary gland
  microarray: suitable
  western blot: suitable
species reactivity   goat, human, rat, bovine, pig
shipped in   dry ice
storage temp.   −20°C
antibody form   ascites fluid
isotype   IgG2b
Quality Level   200
antibody product type   primary antibodies
contains   15 mM sodium azide
UniProt accession no.   Q04695
Gene Information   human ... KRT17(3872)

Description

General description

Cytokeratin 17, with a molecular weight of 46 kDa, is a member of the type I acidic subfamily of cytokeratins that is expressed in normal human epithelial tissues such as glandular epithelium with myoepithelial component, transitional and pseudostratified epithelia. This protein may be involved in the generation of spatial organization of epithelial tissues.

Specificity

Recognizes specifically human cytokeratin 17 (46-47 kDa) and rat cytokeratin 19 (40 kDa). The antibody labels intermediate filaments in human cultured epitheloid carcinoma cell line (HeLa) and in rat hepatoma cells, but does not stain rat fibroblasts in primary cultures. It stains a minor basal cell population in all variants of complex epithelia; strong staining was noted in myoepithelial cells of all glands studied. The antibody exhibits a wide interspecies cross-reactivity, however, the recognized cytokeratin was not studied in cases other than human and rat. The epitope detected by the antibody is sensitive to formalin fixation.

Immunogen

cytoskeletal preparation from rat colon epithelium.

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunohistochemistry (1 paper)

Monoclonal Anti-Cytokeratin Peptide 17 antibody produced in mouse is suitable for western blotting, microarray and indirect immunofluorescence at a working dilution of 1:400 using acetone-fixed frozen sections of human salivary gland. It recognizes an epitope present in epithelial tissues and may be used for typing of normal, metaplastic and neoplastic cells. It may be useful in the discrimination of carcinomas and non-epithelial tumors such as sarcomas, lymphomas and neural tumors. It may also be used for the localization of cytokeratin 17 using various immunochemical assays such as dot blotting and immunohistochemistry. It has been used in the detection of expression of inducible keratin k17 in human keratinocytes in pachyonychia congenita patients.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 2
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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