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C9210 Sigma

Cyanogen bromide-activated Agarose

lyophilized powder



Related Categories Activated / Functionalized Matrices, Activated/Functionalized Matrices, Affinity Chromatography, Antibodies, Antibody Labeling,
form   lyophilized powder
matrix   cross-linked 4% beaded agarose
matrix active group   cyanate or related structures
matrix spacer   1 atom (when ligands are coupled via isourea derivative or related linkage)
swelling   1 g swells to 2.5-4.5 mL
capacity   ≥10 mg/mL binding capacity (BSA)(via amino groups)
storage temp.   2-8°C



1, 5, 15 g in poly bottle

Physical form

Lyophilized powder stabilized with lactose


Cyanogen bromide-activated Agarose is lyophilized powder stabilized with lactose used in affinity chromatography, protein chromatography, protein interactions, antibody labeling, antibody modification and attaching antibodies to agarose beads.

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Safety Information

NONH for all modes of transport
WGK Germany 


Certificate of Analysis

Certificate of Origin

Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis. These documents are located on the product detail page under Useful Links & Tools. Click on the following link to search for a Certificate of Analysis. Please click the following link to see the details on our Product Dating Information.
How do I get lot-specific information or a Certificate of Analysis?
A Certificate of Analysis is available by lot number and can be obtained through our Advanced Search Option: http://www.sigmaaldrich.com/catalog/AdvancedSearchPage.do
What do you recommend to use as a negative control for Product C9210, Cyanogen bromide?
The control for this product is Product No. CL4B200 (Sepharose CL-4B).
What is the difference between Product C9210, Cyanogen bromide and Product C9142, Cyanogen bromide-activated-Sepharose® 4B?
Sigma-Aldrich produces product C9210, while C9142 is produced by GE Healthcare.  In addition, C9210 is a cross-linked resin and C9142 is not.  Please see the product information sheet at this link: http://www.sigmaaldrich.com/etc/medialib/docs/Sigma/Product_Information_Sheet/c9210pis.Par.0001.File.tmp/c9210pis.pdf
When using Product C9210, Cyanogen bromide, why must amine containing reagents be avoided during coupling?
The ligand is attached to the resin via an amine group. If an amine-containing buffer is used during coupling, you will greatly reduce the amount of ligand that can attach.  Please see the product information sheet at this link: http://www.sigmaaldrich.com/etc/medialib/docs/Sigma/Product_Information_Sheet/c9142pis.Par.0001.File.tmp/c9142pis.pdf
How do I find price and availability?
There are several ways to find pricing and availability for our products.  Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers. 
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS. To access the shipping information for this material, use the link on the product detail page for the product, or search here. 
My question is not addressed here, how can I contact Technical Service for assistance?
Use the option to the right to "Ask a Question" by email of a Technical Service Scientist.
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