• USA Home
  • C9281 - Cholesterol Esterase from Pseudomonas fluorescens

C9281 Sigma

Cholesterol Esterase from Pseudomonas fluorescens

lyophilized powder, ≥10,000 units/g protein

Synonym: Sterol-ester acylhydrolase



Related Categories 3.1.x.x Acting on esters, 3.x.x.x Hydrolases, Application Index, Biochemicals and Reagents, Cell Biology,
form   lyophilized powder
composition   Protein, ~20%
solubility   0.4 M potassium phosphate, pH 7.0: soluble 1.0 mg/mL
storage temp.   −20°C


Analysis Note

Protein determined by biuret.

Other Notes

Contains potassium phosphate and TRITON® X-100.


100, 500 units in poly bottle

Unit Definition

One unit will hydrolyze 1.0 μmole of cholesteryl oleate to cholesterol and oleic acid per min at pH 7.0 at 37 °C in the presence of taurocholate.


Cholesterol esterase from Pseudomonas fluorescens has been used in an optimization study of components in enzymatic cholesterol reagents containing cholesterol oxidase. Cholesterol esterase from Pseudomonas fluorescens has also been used in a study to investigate the nondenaturing protein electrotransfer of the esterase activity of lipolytic preparations.

This enzyme is widely used in the determination of serum cholesterol in diagnostic laboratories.

Biochem/physiol Actions

Cholesterol esterase (CE) is a reversible enzyme that can hydrolyze or synthesize fatty acid esters of cholesterol and other sterols. Hydrolysis of water insoluble long chain fatty acid esters requires bile salt activation. Hydrolysis of water soluble esters of short chain fatty acids and lysophospholipids does not require activation by bile salts. It also hydrolyzes tri-, di-, and mono-acylglycerols, phospholipids, lysophospholipids, and ceramides. The enzyme may have multiple functions in lipid and lipoprotein metabolism, as well as in atherosclerosis. Its molecular mass is approximately 129 kDa and the optimum pH range is 7.0-9.0. The enzyme is activated by cholic acid, glycocholic acid, BSA, Mg2+ and 0.3% (v/v) X-100. Ag+, Hg2+ and ionic detergents inhibit the activity of the enzyme.

Legal Information

Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow

Price and Availability

Expore Enzymes, Proteins & Cofactors

Biomedical Applications

Celebrate Pi Day!
Safety & Documentation

Safety Information

NONH for all modes of transport
WGK Germany 
Protocols & Articles


Cholesterol Esterification

To more efficiently transport both dietary and synthesized cholesterol, it is converted to cholesteryl esters. Free cholesterol can be taken up by lipoproteins, but is confined to the outer surface o...
BioFiles 2007, 2.7, 10.
Keywords: Biofiles, Catalysis

Related Content

Enzymes & Proteins

Application Index | Enzyme Index | Substrate Index | Inhibitor Index | Cofactor Index | Lectin Index
Keywords: Cell signaling, Diagnostic, Drug discovery, Molecular biology

Peer-Reviewed Papers


Related Products

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?