C9284 Sigma-Aldrich


pH 5.4(isoelectric point), ammonium sulfate suspension, enzyme from bovine liver

Synonym: H2O2:H2O2 Oxidoreductase



Related Categories Catalase, 1.x.x.x Oxidoreductases, Application Index, Biochemicals and Reagents, Enzyme Class Index,
Quality Level   200
biological source   enzyme from bovine liver
form   ammonium sulfate suspension
mol wt   tetramer ~250 kDa
concentration   ≥800 units/mL
extent of labeling   60,000-120,000 units per g agarose (1900-3800 units per ml of agarose)
optimum pH   ~7.0
matrix   4% cross-linked beaded agarose
pH   5.4(isoelectric point)
storage temp.   2-8°C



Catalase agarose has been used in a study to advance the treatment of oxidative stress in neurodegenerative diseases. Catalase agarose has also been used in a study to investigate the growth of cyanobacterium on agar media in the presence of heterotrophic bacteria.

Catalases have a wide range of industrial applications. The enzyme is used for the removal of traces of (H2O2) that is added to milk and cheese during sterilization. It is used for the degradation of peroxide used in textile bleaching to prevent any problems in subsequent dyeing. Coupled with oxidases, the enzyme is used to prevent the inactivation of oxidases by the action of high concentration of peroxide. It is also used to prevent side reactions caused by (H2O2) that can degrade the target product.

Biochem/physiol Actions

Catalase activity is constant over the pH range of 4.0-8.5. Each subunit of the tetramer contains iron bound to a protoheme IX group. The enzyme also strongly binds NADP, which is in close proximity to the heme group. The enzyme activity is inhibited by 3-amino-1-H-1,2,4 triazole, cyanide, azide, hydroxylamine, cyanogen bromide, 2-mercaptoethanol, dithiothreitol, dianisidine, and nitrate. Incubation of catalase with ascorbate or ascorbate/Cu2+ results in degradation of the catalase molecule.


Freezing of solutions is not recommended.

Unit Definition

One unit will decompose 1.0 μmole of H2O2 per min at pH 7.0 at 25 °C, while the H2O2 concentration falls from 10.3 to 9.2 mM, measured by the rate of decrease of A240.

Physical form

Suspension in 3.2 M (NH4)2SO4, pH 7, containing 0.02% thimerosal

Safety & Documentation

Safety Information

NONH for all modes of transport
WGK Germany 
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable


Certificate of Analysis (COA)

Please Enter a Lot Number

Certificate of Origin (COO)

Please Enter a Lot Number
Protocols & Articles

Related Content

Enzymes & Proteins

Committed to developing and providing breakthrough problem-solving reagents and solutions, explore our comprehensive offering of enzymes and protein reagents and kits with the confidence that you wil...
Keywords: Cell signaling, Diagnostic, Enzyme-linked immunosorbent assay, Immunohistochemistry, Metabolism, Molecular biology, Purification, Western blot

Peer-Reviewed Papers


Related Products


Product #


Add to Cart

A8056 3-Amino-1,2,4-triazole, ≥95% (TLC)

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?