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C9301 Sigma-Aldrich

Collagen from chicken sternal cartilage

Type II (Miller), powder, BioReagent, suitable for cell culture

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Properties

Related Categories Adhesion, Attachment, and Matrix Factors for Stem Cell Expansion, Application Index, Attachment Factors, Attachments Factors, Biochemicals and Reagents,
Quality Level   PREMIUM
biological source   chicken (Sternal cartilage)
sterility   non-sterile
type   Type II (Miller)
product line   BioReagent
form   powder
packaging   glass bottle of 100 mg
  poly bottle of 25 mg
  glass bottle of 5 mg
surface coverage   6‑10 μg/cm2
solubility   acetic acid: 0.5-2.0 mg/mL (Dissolve for several hours at 2-8 °C, occasionally swirling.)
suitability   suitable for cell culture
storage temp.   2-8°C

Description

General description

Collagen is made of three polypeptide chains. Type 2 collagen is ubiquitously present in the hyaline cartilage. It is a homotrimer made of three α1 chains. Type 2 collagen is also predominant in nucleus pulposus of the vertebral disc and vitreous body of the eye. The proteoglycan obtained from the chicken sternal cartilage is similar to type nine collagen structure.

Collagen is classified into a number of structurally and genetically distinct types. We use the nomenclature proposed by Bornstein and Traub. Do not confuse Sigma type designations with recognized collagen classification types.

Application

Collagen from chicken sternal cartilage has been used in enzyme–linked immunosorbent assay (ELISA) , chondrocyte-mediated tissue production in vitro, induction, treatment and assessment of collagenα induced arthritis (CIA) and cell proliferation assay.

Packaging

100 mg in glass bottle

25 mg in poly bottle

5 mg in glass bottle

Other Notes

Major component of articular cartilage.

Preparation Note

Prepared by a modification of the method of Trentham, D.E., et al.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
1

Documents

Certificate of Analysis

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Certificate of Origin

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Cell Culture Guide
Protocols & Articles

Articles

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Collagen Attachment Protocols, Solubility, and Stability

A1) We recommend transferring the collagen solution to a glass bottle with a screw cap and carefully layering chloroform at the bottom. The amount of chloroform to use should be ~10% of the volume of...
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Keywords: Centrifugation, Dialysis, Filtration, Purification, Sterilizations

Collagen Usage Recommendations

Collagen is one of the most abundant proteins in the connective tissues and internal organs of mammals. It provides the tensile strength of the extracellular matrix (ECM). It is classified into a num...
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BioFiles 2008, 3.8, 4.
Keywords: Cell culture, Morphogenesis

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Animal cells and tissue culture techniques are constantly improved to optimize in vitro cell culture conditions. Extracellular Matrix (ECM) proteins coating, chemical or physical modification of the ...
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What is the Extracellular Matrix?

The extracellular matrix (ECM) is secreted by cells and surrounds them in tissues. It has long been understood to be the structural support for cells since its characteristics set the characteristics...
Keywords: Adhesion, Cell culture, Cellular processes, Epimerizations, Growth factors, Immobilization, Sulfations

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Peer-Reviewed Papers
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References

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