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C9409 Sigma-Aldrich

Creatinine Deiminase microbial

lyophilized powder, ≥25 units/mg protein

Synonym: Creatinine Deaminase

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Properties

Related Categories 3.5.x.x Acting on C-N other than peptides, 3.x.x.x Hydrolases, Biochemicals and Reagents, Cell Biology, Enzyme Class Index,
Quality Level   200
form   lyophilized powder
specific activity   ≥25 units/mg protein
mol wt   ~260 kDa
composition   Protein, ≥15% biuret
storage temp.   −20°C

Description

Application

Creatinine deiminase has been used in a study to assess the application of a creatinine-sensitive biosensor for hemodialysis control. Creatinine deiminase has also been used in a study to investigate the bioelectronic tongue for the simultaneous determination of urea, creatinine and alkaline ions in clinical samples.

Packaging

250 units in poly bottle

Physical properties

Isoelectric point : 4.4
Michaelis constant : 3.5 x 10‾3M (Creatinine)
Structure : 6 subunits per mol of enzyme
Inhibitors : Ag+,Hg++, o-phenanthroline,monoiodoacetate
Optimum pH : 8.5 – 9.5
Optimum temperature : 65 – 75°C
pH Stability : pH 7.0 – 11.0 (30°C, 20hr)
Thermal stability : Below 65°C (pH 7.5, 1hr)

Unit Definition

One unit will hydrolyze 1.0 μmole of creatinine to N-methylhydantoin and NH3 per min at pH 7.5 at 37 °C in a coupled system with L-glutamic dehydrogenase.

Physical form

Lyophilized powder containing mannitol as stabilizer

Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 3
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Protocols & Articles

Protocols

Assay Procedure for Creatinine Deiminase Microbial

GIDH:Glutamate dehydrogenase(NADP+ dependent)[L-Glutamate:NADP oxidoreductase (deaminating), (EC 1.4.1.4.)] The disappearance of NADPH is measured at 340nm by spectrophotometry.
Keywords: Extinction coefficient, Oxidations

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Peer-Reviewed Papers
15

References

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