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C9491 Sigma-Aldrich

Anti-CtBP1, N-terminal antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

Synonym: Anti-Carboxy-terminal binding protein 1

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies against Proteins, Bioactives, Markers and Receptors, Antibodies for Cell Biology, Antibodies for Epigenetics and Nuclear Signaling,
conjugate   unconjugated
clone   polyclonal
biological source   rabbit
application(s)   immunoprecipitation (IP): 5-10 μL using lysates of K562 cell line
  microarray: suitable
  western blot: 1:250-1:500 using mouse brain lysates
  western blot: 1:500-1:1,000 using K562 cells
species reactivity   human, mouse
mol wt   antigen 48 kDa
form   buffered aqueous solution
shipped in   dry ice
storage temp.   −20°C
antibody form   IgG fraction of antiserum
antibody product type   primary antibodies
UniProt accession no.   Q13363
Gene Information   human ... CTBP1(1487)
mouse ... Ctbp1(13016)

Description

Immunogen

synthetic peptide corresponding amino acids 2-18 of human CtBP1. This sequence is conserved in mouse and does not cross-react with CtBP2.

Application

Anti-CtBP1, N-terminal antibody produced in rabbit is suitable for immunoprecipitation and immunoblotting at a working dilution of 1:500-1:1000 using cell lysates of the K562 cell line and 1:250-1:500 using mouse brain lysates.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Biochem/physiol Actions

Apart from E1A protein, CtBP1 interacts with a wide variety of cellular factors such as DNA-binding transcription factors that participate in several developmental biological pathways and processes by binding to the carboxy terminal. It also interacts with polycomb group proteins and thus a component of the chromatin remodeling machinery and transcriptional repression. Pnn/DRS, a phosphoprotein involved in the regulation of cell adhesion and modulation of the activity of multiple tumor supressor genes, interacts with CtBP1 and may modulate repression of E-cadherin transcription. CtBP1 associates with SMAD6 and represses BMP signaling of the TGF-β pathway in the nucleus. It acts as transcriptional coregulator in histone acetyltransferase or histone deacetylase (HDAC)-dependent and independent manner.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

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Milli-Q® Water Purification Solutions
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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