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C9658 Sigma-Aldrich

Carboxypeptidase G from Pseudomonas sp.

lyophilized powder, ≥3 units/mg protein

Synonym: γ-Glutamyl hydrolase

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Description

Application

Carboxypeptidase G from Pseudomonas sp., or γ-Glutamyl hydrolase, has been used in a study to assess the role of the putidaredoxin COOH-terminus in P-450cam (cytochrome m) hydroxylations. Carboxypeptidase G from Pseudomonas sp. has also been used in a study to investigate the effects of nitric oxide on pemetrexed cytotoxicity via NO‑cGMP signaling in lung adenocarcinoma cells.

Packaging

20 units in glass bottle

5 units in poly bottle

Biochem/physiol Actions

Carboxypeptidase G is a lysosomal, thiol-dependent protease, which progressively cleaves γ-glutamyl pteroyl poly-γ-glutamate yielding pteroyl-α-glutamate (folic acid) and free glutamate. It is considered highly specific for the γ-glutamyl bond, but not for the C-terminal amino acid of the leaving group. Molecular mass of this homodimer is approximately 90 kDa. The enzyme is activated by Zn2+ ions.

Unit Definition

One unit will hydrolyze 1.0 μmole of L-glutamic acid from (+)amethopterin per min at pH 7.3 at 30 °C.

Physical form

Contains sodium acetate salt.

Preparation Note

Chromatographically purified

Solutions should be prepared fresh prior to use.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

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Certificate of Origin

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Protocols & Articles

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Enzymes & Proteins

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Keywords: Cell signaling, Diagnostic, Drug discovery, Metabolomics, Molecular biology

Peer-Reviewed Papers
15

References

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