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C9672 Sigma-Aldrich

Monoclonal Anti-Neural Cell Adhesion Molecule antibody produced in mouse

clone NCAM-0B11, ascites fluid

Synonym: Anti-CD56-CY, Anti-NCAM

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Properties

Related Categories Alphabetical Index, Analytical Cytology, Antibodies, Antibodies for Cell Biology, Antibodies for Immune Cell Signaling and Blood,
conjugate   unconjugated
clone   NCAM-0B11, monoclonal
biological source   mouse
application(s)   immunocytochemistry: suitable using cells and tissues
  western blot: 1:100 using a freshly prepared extract from newborn and adult rat brain, or rat cerebral cortex extract
species reactivity   rat, human
mol wt   antigen 140-180 kDa
shipped in   dry ice
storage temp.   −20°C
antibody form   ascites fluid
isotype   IgG1
Quality Level   200
antibody product type   primary antibodies
contains   15 mM sodium azide
UniProt accession no.   O15394
  P13591
Gene Information   human ... NCAM1(4684), NCAM2(4685)
rat ... Ncam1(24586), Ncam2(288280)

Description

General description

Monoclonal Anti-N-CAM (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Neural cell adhesion molecule (N-CAM), the best characterized CAM, exists in adult brain. It belongs to the family of sialoglycoproteins, which arise from alternative splicing of mRNA transcribed from a single gene. Immunocytochemical data indicate that in the adult, N-CAM is expressed mainly in cells of the nervous system. There are indications that N-CAM may also be expressed by non-neural cells in the adult, as it has been found in studies of various embryonic developmental stages.

Mouse monoclonal clone NCAM-0B11 anti-Neural Cell Adhesion Molecule antibody localizes the high molecular weight isoform of N-CAM (neural cell adhesion molecule) in human and several other mammalian species. The antibody shows a strong reaction to N-CAM A/N-CAM 180, however it also recognizes N-CAM B/N-CAM 140. In an immunoblot, the product reacts with polysialated N-CAM in embryonic material. Breakdown products of N-CAM may be stained; they appear as bands lower than 100 kD on the immunoblot.

Immunogen

growth cone enriched plasma membrane fraction from E17 rat forebrain.

Application

Monoclonal Anti-Neural Cell Adhesion Molecule antibody produced in mouse has been used in:
• Immunofluorescence
• Immunohistochemistry
• Immunoblotting

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biochem/physiol Actions

Neural cell adhesion molecule (N-CAM) are believed to be involved in cell-cell interactions and probably play an important role in embryogenesis and development.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
nwg
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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