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C9791 Sigma

Collagen from calf skin

Bornstein and Traub Type I, solid, BioReagent, suitable for cell culture



Related Categories Adhesion, Attachment, and Matrix Factors, Adhesion, Attachment, and Matrix Factors for Stem Cell Expansion, Attachment Factors, Attachments Factors, Biochemicals and Reagents,
product line   BioReagent
form   solid
availability   not available in Canada (at this time; for questions or status updates, please email us at antibody.canada@sial.com)
surface coverage   6‑10 μg/cm2
solubility   0.1 M acetic acid: 1 mg/mL (Allow to stir at room temperature 1-3 hours until dissolved.)
suitability   suitable for cell culture
storage temp.   2-8°C



All collagen molecules are composed of three polypeptide chains arranged in a triple helical conformation, with a primary structure that is mostly a repeating motif with glycine in every third position and proline or 4-hydroxyproline frequently preceding the glycine residue. Type I collagen differs from other collagens by its low lysine hydroxylation and low carbohydrate composition.


This product is intended to produce thin layer coatings on tissue culture plates to facilitate attachment of anchorage-dependent cells, recommended for use at 6-10 μg/cm2. It is NOT intended for production of 3-D gels. Type I collagen is often used in cell culture as an attachment substratum with myoblasts, spinal ganglia, hepatocytes, embryonic lung, heart explants, fibroblasts, endothelial cells, and islet cells have all been cultured successfully on films or gels of type I collagen. Collagen type I may also be used in research of Idiopathic pulmonary fibrosis (IPF), studies on the effect of ER stress IPF on lung fibroblasts. Collagen in acidic solution can produce three dimensional scaffolding with use in bioengineering and cell culture applications.

Biochem/physiol Actions

Type I collagen is a component of skin, bone, tendon, and other fibrous connective tissues.

Other Notes

Collagen is classified into a number of structurally and genetically distinct types. We use the nomenclature proposed by Bornstein and Traub. Do not confuse Sigma type designations with recognized collagen classification types.


10, 50, 100, 250 mg in poly bottle

Preparation Note

This product was prepared by a modification of Gallop, P.M. and Seifter, S., Meth. Enzymol., VI, 635 (1963). It is soluble at 1 mg/mL in .1 M acetic acid and should be stirred at room temperature for 1-3 hours until dissolved.

Price and Availability

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Safety & Documentation

Safety Information

NONH for all modes of transport
WGK Germany 


Certificate of Analysis

Certificate of Origin

Protocols & Articles


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Keywords: Cancer, Cell attachment, Cell culture, Cell proliferation, Gastrointestinal, Gene expression, Transcription, transformation

Collagen Attachment Protocols, Solubility, and Stability

A1) We recommend transferring the collagen solution to a glass bottle with a screw cap and carefully layering chloroform at the bottom. The amount of chloroform to use should be ~10% of the volume of...
George Sitterley
BioFiles 2008, 3.8, 5.
Keywords: Centrifugation, Dialysis, Filtration, Purification, Sterilizations

Collagen Usage Recommendations

Collagen is one of the most abundant proteins in the connective tissues and internal organs of mammals. It provides the tensile strength of the extracellular matrix (ECM). It is classified into a num...
George Sitterley
BioFiles 2008, 3.8, 4.
Keywords: Cell culture, Morphogenesis

Extracellular Matrix Proteins and tools for cell culture optimization

Animal cells and tissue culture techniques are constantly improved to optimize in vitro cell culture conditions. Extracellular Matrix (ECM) proteins coating, chemical or physical modification of the ...
Keywords: Adhesion, Angiogenesis, Apoptosis, Asymmetric synthesis, Cancer, Cell attachment, Cell culture, Cell proliferation, Coagulation, Endocrinology, Growth factors, Hormones

What is the Extracellular Matrix?

The extracellular matrix (ECM) is secreted by cells and surrounds them in tissues. It has long been understood to be the structural support for cells since its characteristics set the characteristics...
Keywords: Adhesion, Cell culture, Cellular processes, Epimerizations, Growth factors, Immobilization, Sulfations

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