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C9830 Sigma

Chitosanase from Streptomyces griseus

lyophilized powder, >50 units/mg protein (Bradford)

Synonym: Chitosan N-acetylglucosaminohydrolase



Related Categories 3.2.x.x Glycosidases, 3.x.x.x Hydrolases, Application Index, Biochemicals and Reagents, Carbohydrate Hydrolysis,
form   lyophilized powder
purified by   chromatography
solubility   H2O: soluble, clear to slightly hazy, colorless (10U/0.3mL)
foreign activity   chitinase <1.0 unit/mg protein
storage temp.   −20°C


Other Notes

View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer


10 units in serum bottle

Preparation Note

For long term solution storage, reconstitute vial with 50% glycerol solution. Dilute to working concentrations with a 0.1% BSA solution.

Unit Definition

One unit will release 1 μmole of glucosamine from chitosan per min at pH 5.0 at 37 °C as measured in the fluorimetric assay of Osswald, et al. (1992).

Physical form

Lyophilized powder containing potassium phosphate buffer salts


Chitosanase from Streptomyces griseus has been used in a study to assess the effect of chitin sources on production of chitinase and chitosanase. Chitosanase from Streptomyces griseus has also been used in a study to investigate the effective production of chitinase and chitosanase by Streptomyces griseus HUT 6037 using colloidal chitin and various degrees of deacetylation of chitosan.

The enzyme from Sigma has been used for the hydrolysis of the crude cell wall preparation of oomycete Aphanomyces euteiches, a major parasite of legume plants. It has also been used for the enzymatic hydrolysis of the fully de-N-acetylated chitosan to get chitosan oligomer mixtures during the preparation of biocompatible chitosan-alginate gel.

Biochem/physiol Actions

Chitosanase catalyzes the endohydrolysis of β(1,4) linkages between N-acetyl-D-glucosamine and D-glucosamine residues in partially deacetylated chitosan. Chitosanase from Streptomyces griseus is capable of hydrolyzing both chitosan and carboxymethyl cellulose. It is used for the lysis of cell walls of fungi belonging to the group Mucorales. It is found in several types of microorganisms.

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Safety & Documentation

Safety Information

NONH for all modes of transport
WGK Germany 
Protocols & Articles


Enzymatic Assay of Chitosanase from Streptomyces Species

This procedure applies to Sigma-Aldrich Product Number, C0794. This procedure is NOT to be used to assay Chitosanase from Streptomyces griseus, Sigma-Aldrich Product Number, C9830.
Keywords: Biochemistry, Enzyme activity, Microbiology

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Application Index | Enzyme Index | Substrate Index | Inhibitor Index | Cofactor Index | Lectin Index
Keywords: Cell signaling, Diagnostic, Drug discovery, Molecular biology

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