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CA200 Sigma-Aldrich

cAMP Enzyme Immunoassay Kit, Direct

  •  NACRES NA.84

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Description

General description

Adenosine 3′,5′-cyclic monophosphate (cAMP) is a second messenger in many physiological processes. It is synthesized from adenosine triphosphate (ATP) in the presence of enzyme adenylate cyclase.

Application

cAMP Enzyme Immunoassay Kit, Direct has been used to measure cAMP levels in cell culture medium virulent and attenuated macrophages. It has also been used to quantify cAMP synthesis in olfactory epithelium.

Biochem/physiol Actions

Adenosine 3′,5′-cyclic monophosphate (cAMP) is shown to be involved in the cardiovascular and nervous systems, immune mechanisms, cell growth and differentiation, and general metabolism. Deciphering cAMP modulation in regulatory pathways may help in understanding of the physiology and pathology of many disease states.

Other Notes

Allow reagents to warm at room temperature for 30 minutes prior to opening.

Kit component only

Description

Product #

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0.1 M Hydrochloric Acid 1 x 30 mL    
Acetic Anhydride 1 x 1 mL    
cAMP-Alkaline Phosphatase Conjugate 1 x 5 mL    
cAMP EIA Antibody Rabbit Anti-cAMP 1 x 5 mL    
5 Cycle Logit-Log Paper 1 x 1 sheets    
Cyclic AMP Standard 1 x 0.5 mL    
Direct cAMP Assay Layout Sheet 1 x 1 sheets    
Goat Anti-Rabbit IgG Coated 96 Well Microtiter Plate 1 ea    
Neutralizing Reagent 1 x 6 mL    
p-Nitrophenyl Phosphate Substrate Solution 1 x 20 mL    
Plate Sealer 1 ea    
Stop Solution 1 x 5 mL    
Triethylamine 1 x 2 mL    
Wash Buffer Concentrate 1 x 30 mL    
Safety & Documentation

Safety Information

Symbol 
Signal word 
Danger
Precautionary statements 
RIDADR 
UN 3316 9
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

ELISA Protocols | Sandwich ELISA Protocol & More Types

NOTE: ALL incubations and wash steps must be performed under gentle rocking or rotation (~1‐2 cycles/sec).
Keywords: Cell culture, Phosphorylations, Size-exclusion chromatography

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Peer-Reviewed Papers
15

References

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