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Sigma

SAF-1

00122301

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Properties

Related Categories Cell Culture, ECACC Cell Lines, S-Z More...
biological source   fish fin
growth mode   Adherent
karyotype   Not specified
morphology   Fibroblast-like
products   Not specified
receptors   Not specified
application(s)   cell culture | mammalian: suitable
shipped in   dry ice
storage temp.   −196°C

Description

Other Notes

Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.

Depositor and originator: M. Carmen Alvarez and J.Jose Borrego. Universidad de Malaga, Fac de Ciencias, Campus de Teatinos, Malaga, 29071

Application

Genetic engineering, virus susceptibility, fish physiology.

Cell Line Origin

Gilt head seabream caudal fin

Culture Medium

L15 (L5520) + 4mM L-Glutamine (G7513) + 15% FBS / FCS (F2442)

Subculture Routine

Split sub-confluent cultures (70-80%) 1:3 -1.6 ie. seeding at 2-4 x10,000 cells/cm2 using 0.25% trypsin/EDTA; 25°C; no CO2.

Cell Line Description

SAF-1 has been developed from the fin tissues of an adult gilt-head seabream (sparius aurata) without immortalising treatments. SAF-1 supports the replication of several common fish viruses such as lymphocystis disease virus (LDV), infectious haematopoietic necrosis virus (IHNV) and viral haemorrhagic septicaemia virus (VHSV). SAF-1 has a higher susceptibility to rhabdoviruses, especially UHNV. In the SAF-1 line, neither chromosome changes or differences from passage 50-70 have been detected, suggesting a normal karyotype and stable cell line. The cell line may be useful in isolating and studying undetected latent and potential lytic viruses infecting gilt-head seabream and other related sparid species. In addition to its proven applications, SAF-1 could be used as an in vitro system to address multiple questions related to the genetic engineering of this species, such as evaluating the efficiency of the promotors in transgenic constructions, testing whether this species is susceptible to viral vectors available in fish or whether heterologous transposable elements can be mobilised for insertional mutagenesis. This cell line was mycoplasma eradicated at ECACC.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis


Cell Culture Guide

ECACC Laboratory Handbook
Protocols & Articles
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