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Sigma

tsDC

from mouse bone, 01081609

Synonym: TSDC

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Properties

Related Categories Cell Culture, ECACC Cell Lines, S-Z More...
biological source   mouse bone
growth mode   Semi-adherent
karyotype   Not specified
morphology   Not specified
products   Not specified
receptors   Not specified
  Not specified
application(s)   cell culture | mammalian: suitable
shipped in   dry ice

Description

Other Notes

Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.

Legal Information

This material is cited in US and/or other patent and may not be used to infringe patent claims. US patent No′s. 5688692, 5866759 and international patent pending PCT/GB91/00262.

Cell Line Origin

Immortalised mouse dendritic cell line

DNA Profile

Not specified

Culture Medium

IDMEM (I3390) + 2 mM L-Glutamine (G7513) + 5% FBS (F2442) + 100U/ml Penicillin + 100 ug/ml Streptomycin + 0.05 mM 2ME (M6250)

Subculture Routine

Passage cells approximately once every 2 weeks. Culture at 33 °C; 5-10% CO2. Cells are of heterogenous morphology, but the line has been cloned. Some cells adhere and others grow in suspesion. Cells are detached by short incubation in PBS plus 20mM EDTA, followed by washing in culture medium. Despite the cells slow growth, they can be highly diluted -growth does not depend on a minmum cell density.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis


Cell Culture Guide

ECACC Laboratory Handbook
Protocols & Articles
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