EMAIL THIS PAGE TO A FRIEND
Sigma

10(AAT) Clone A

02043015, Reactivity: Anti-α1-Antitrypsin (also known as α1-AT or AAT)

Purchase

Properties

Related Categories Cell Culture, ECACC Cell Lines, Hybridoma Collection More...
biological source   Mouse x Mouse Hybridoma
ImmunoDon   BALB/c mouse spleen
Myeloma   Sp2/0
Reactivity   Anti-α1-Antitrypsin (also known as α1-AT or AAT)
application(s)   cell culture | mammalian: suitable
isotype   IgG1
shipped in   dry ice
storage temp.   −196°C

Description

Cell Line Origin

Mouse Sp2/0 x Mouse BALB/c spleen

Cell Line Description

The antibody is directed against α1-antitrypsin (also known as α1-AT or AAT).

Immunogen

Various peptides between 9 and 11 amino acids attached to PPD via a terminal cysteine residue

Application

ELISA, lateral flow tests, western blot analysis

Culture Medium

EMEM + 2mM glutamine + 10 - 15% Foetal Bovine Serum (FBS)

Subculture Routine

Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C. Split ratio: 1:2

Other Notes

Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis (COA)

Please Enter a Lot Number

Pipette Controller from Brand
Protocols & Articles
Related Products

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?