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Sigma

Vero/hSLAM

04091501, from African green monkey kidney, Convention on International Trade in Endangered Species of Wild Fauna and Flora (CITES)

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Properties

Related Categories CITES, Cell Culture, ECACC Cell Lines, S-Z
biological source   African green monkey kidney
growth mode   Adherent
karyotype   Not specified
morphology   Fibroblast
products   Not specified
receptors   SLAM; also known as CDw150, a receptor for measles virus
application(s)   cell culture | mammalian: suitable
shipped in   dry ice
storage temp.   −196°C

Description

Other Notes

Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.

Cell Line Origin

Monkey African Green Kidney human CDw150 (SLAM) mealses virus receptor expression

Culture Medium

MEM with Earle's salts + 2 mM L-Glutamine (G7513) + 5% FBS / FCS (F2442) + 0.4 mg/ml Geneticin. It is essential that geneticin is added to the culture medium as it is required to maintain the expression of the SLAM gene.

Subculture Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:4, i.e., seeding at 1-3x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37 °C.

Cell Line Description

Vero/hSLAM was derived by transfection of Vero cells with a vector plasmid (pCXN2) containing the neomycin resistance gene and an expression plasmid (pCAG-hSLAM) encoding the human signaling lymphocytic activation molecule (SLAM) , also known as CDw150, which is a receptor for measles virus. Clones of transfected cells were selected in the presence of geneticin (also known as G418). Vero/hSLAM cells can be used to isolate measles virus from human clinical samples and are a substitute for the B95a cell line for this purpose. Mumps susceptibility has also been demonstrated at the depositor′s laboratory.

Safety & Documentation

Safety Information

RIDADR 
UN 3245 9
WGK Germany 
3

Documents

Certificate of Analysis


Cell Culture Guide

ECACC Laboratory Handbook
Protocols & Articles
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