Sf9 TitreHigh AC free




Related Categories Cell Culture, ECACC Cell Lines, S-Z More...
biological source   insect ovary
growth mode   Adherent
karyotype   Not specified
morphology   Spherical
products   Not specified
receptors   Not specified
application(s)   cell culture | mammalian: suitable
shipped in   dry ice
storage temp.   −196°C


Cell Line Origin

Spodoptera frugiperda pupal ovarian tissue, serum-free

Cell Line Description

This cell line is a derivative of the Sf9 cell line which has been adapted to TiterHigh Sf Insect Medium (Sigma I5408). TiterHigh Sf Insect Medium is an animal component-free, protein-free formulation designed specifically for the Sf9 (Spodoptera frugiperda) insect cell line. The cell line Sf9 is derived from pupal ovarian tissue of spodoptera frugiperda. The Sf9 cell line is highly susceptible to Baculovirus infection and is used in the production of protein products genetically manipulated into Baculovirus vector systems.


Virus studies: Baculovirus

Culture Medium

TiterHigh Sf Insect Medium (Sigma I5408) no supplements.

Subculture Routine

Maintain cultures between 3x100,000 to 1x1,000,000 cells/ml; 27°C. Gently resuspend cells in old culture medium by dislodging the cells by pipetting, or gentle agitation, and resuspend in fresh medium. Cells should stick to the culture flask. Freeze in 7.

Other Notes

Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.

Depositor and originator: Dr. E. Penn, European Collection of Cell Cultures, Health Protection Agency, Porton Down, Salisbury, SP4 0JG, UK

Legal Information

TiterHigh is a trademark of Sigma-Aldrich Co. LLC

Safety & Documentation

Safety Information

NONH for all modes of transport


Certificate of Analysis


Cell Culture Guide

ECACC Laboratory Handbook
Protocols & Articles
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