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Sigma

Sf21

05022801

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Properties

Related Categories Cell Culture, ECACC Cell Lines, S-Z More...
biological source   insect ovary
growth mode   Adherent
karyotype   Not specified
morphology   Spherical
products   Not specified
receptors   Not specified
application(s)   cell culture | mammalian: suitable
shipped in   dry ice
storage temp.   −196°C

Description

Other Notes

Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.

Application

Protein expression using the Baculovirus vector system

Cell Line Origin

Spodoptera frugiperda pupal ovarian tissue

Culture Medium

SF90011 + 10% Foetal Bovine Serum (FBS)

Subculture Routine

Split sub-confluent cultures (70-80%) 1:2 i.e. seeding at 5x10,000 cells/cm2; 28 C. Gently resuspend cells in old culture medium by dislodging the cells by pipetting, or gentle agitation, centrifuge suspension and resuspend cell pellet in fresh medium.

Cell Line Description

Derived from pupal ovarian tissue of spodoptera frugiperda. The cells are highly susceptible to Baculovirus infection and are used in the production of protein products genetically manipulated into Baculovirus vector systems. They were derived at the USDA Insect Pathology Laboratory from a cell line originating from pupal ovarian tissue of the fall army worm, Spodoptera frugiperda. Sf21 cells have greater range in size and have a doubling time of 24 hr compared to 48-72 hr for Sf9 cells.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis


Cell Culture Guide

ECACC Laboratory Handbook
Protocols & Articles
Related Products

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