Related Categories Cell Culture, ECACC Cell Lines, S-Z More...
biological source   human bladder
growth mode   Not specified
karyotype   Aneuploid, with specific marker chromosomes
morphology   Epithelial
products   Not specified
receptors   Not specified
application(s)   cell culture | mammalian: suitable
relevant disease(s)   cancer
shipped in   dry ice
storage temp.   −196°C


Other Notes

Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.

Depositor and originator: Prof H Hameister, Dept of Human Genetics, University of Ulm, D-89010, Ulm, Germany

Cell Line Origin

Human bladder transitional cell carcinoma

Culture Medium

2/3 DMEM:1/3 RPMI + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:2 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C. The medium must be changed to fresh medium every 48 hours.

Cell Line Description

Spontaneously immortal cell line established from a primary transitional cell carcinoma of the bladder. Derived from a female patient.

Safety & Documentation

Safety Information

NONH for all modes of transport


Certificate of Analysis

Cell Culture Guide

ECACC Laboratory Handbook
Protocols & Articles
Related Products

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