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Sigma

WERI

06070602

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Properties

Related Categories Cell Culture, ECACC Cell Lines, S-Z More...
biological source   human eye
growth mode   Semi-adherent
karyotype   Not specified
morphology   Grape like clusters of round cells
products   Not specified
receptors   Not specified
application(s)   cell culture | mammalian: suitable
relevant disease(s)   cancer
shipped in   dry ice
storage temp.   −196°C

Description

Cell Line Origin

Human Retinoblastoma

Cell Line Description

Human retinoblastoma cell line derived from a female Caucasian. The cells are semi-adherent, some neuronal differentiation can occur when subculturing. Tumourigenic in rabbits.

Culture Medium

Iscove′s Modified DMEM + 2mM Glutamine + 15% Foetal Bovine Serum (FBS) + 10ug/ml Insulin + 55uM 2-Mercaptoethanol

Subculture Routine

Split subconfluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 2-3 x100,000 cells/ml; 5% CO2; 37°C. Saturation density: 2 x 1000,000 cells/ml. Cells are semi-adherent. Scrape cells from surface; do not use trypsin.

Other Notes

Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis

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Cell Culture Guide

ECACC Laboratory Handbook
Protocols & Articles
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