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Sigma

1156QE/8

06072609

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Properties

Related Categories 0-10, Cell Culture, ECACC Cell Lines More...
biological source   human embryo (carcinoma)
growth mode   Not specified
karyotype   Not specified
morphology   Not specified
products   Not specified
receptors   Not specified
application(s)   cell culture | mammalian: suitable
relevant disease(s)   cancer
shipped in   dry ice

Description

Cell Line Origin

Human embryonal carcinoma

Cell Line Description

A human embryonal carcinoma cell line established from a primary testicular tumour of a 22 year-old patient. By short tandem repeat (STR)-PCR analysis the Y chromosome could not be detected in this cell line when tested at ECACC. It is a known phenomenon that due to the increased genetic instability of cancer cell lines the Y chromosome can be rearranged or lost resulting in lack of detection.

DNA Profile

STR-PCR Data:

Amelogenin: X
CSF1PO: 11
D13S317: 8,9
D16S539: 11,14
D5S818: 11,12
D7S820: 9
THO1: 9.3
TPOX: 8
vWA: 16,17

Culture Medium

DMEM (High Glucose) + 2 mM Glutamine + 10% Fetal Bovine Serum (FBS). No sodium pyruvate.

Subculture Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:5 i.e. seeding at >6x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 10% CO2; 37°C. Cells need to be maintained at a high cell density.

Other Notes

Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis (COA)

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Protocols & Articles
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