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Sigma

UM-UC-1

06080301

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Properties

Related Categories Cell Culture, ECACC Cell Lines, S-Z More...
biological source   human kidney (renal gland)
growth mode   Adherent
karyotype   Not specified
morphology   Epithelial
products   Not specified
receptors   Not specified
application(s)   cell culture | mammalian: suitable
relevant disease(s)   metastasis
shipped in   dry ice
storage temp.   −196°C

Description

Other Notes

Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.

Depositor and originator: Dr H Barton Grossman, Department of Urology, University of Texas, M.D. Anderson Cancer Center, 1515 Holcombe Boulevard, Texas 77030 USA

Cell Line Origin

Human bladder transitional cell carcinoma

DNA Profile

STR-PCR Data: Amelogenin: X
CSF1PO: 12
D13S317: 12,14
D16S539: 9,11
D5S818: 13
D7S820: 8,11
THO1: 8
TPOX: 8
vWA: 14,15

Culture Medium

MEM + 1% NEAA + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Split subconfluent cultures (70-80%) 1:4 to 1:10 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C. Doubling time approximately 26 hours.

Cell Line Description

Human transitional cell carcinoma (grade II) derived from a bladder cancer metastasis of a black male. Metastatic involvement of lymphatics. By short tandem repeat (STR)-PCR analysis the Y chromosome could not be detected in this cell line when tested at ECACC. It is a known phenomenon that due to the increased genetic instability of cancer cell lines the Y chromosome can be rearranged or lost resulting in lack of detection.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis


Cell Culture Guide

ECACC Laboratory Handbook
Protocols & Articles
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