Vero (AC-free)

08011101, from African green monkey kidney, Convention on the International Trade in Endangered Species of Wild Fauna and Flora (CITES)



Related Categories CITES, Cell Culture, ECACC Cell Lines, S-Z
biological source   African green monkey kidney
growth mode   Adherent
karyotype   Not specified
morphology   Epithelial
receptors   Not specified


Other Notes

Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.

Depositor and originator: Dr. E. Penn, European Collection of Cell Cultures, Health Protection Agency, Porton Down, Salisbury,

Cell Line Origin

African green monkey kidney cells, serum-free

Culture Medium

VP-SFM (Gibco cat no. 11681-020) + 4 mM L-Glutamine (G7513). When freezing cells down use culture medium (50:50 conditioned medium:fresh medium) + 10% DMSO (D2650).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:6, i.e., seeding at 1-3x10,000 cells/cm2 using 0.05% trypsin or trypsin/EDTA; 5% CO2; 37 °C.

Cell Line Description

Vero cell line (Sigma catalog no. 84113001) adapted to grow in animal component free medium (VP-SFM; Gibco catalog no. 11681-020).

Safety & Documentation

Safety Information

WGK Germany 


Certificate of Analysis

Cell Culture Guide

ECACC Laboratory Handbook
Protocols & Articles
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