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Sigma

Vero (AC-free)

08011101, from African green monkey kidney, Convention on the International Trade in Endangered Species of Wild Fauna and Flora (CITES)

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Properties

Related Categories CITES, Cell Culture, ECACC Cell Lines, S-Z
biological source   African green monkey kidney
growth mode   Adherent
karyotype   Not specified
morphology   Epithelial
receptors   Not specified

Description

Cell Line Origin

African green monkey kidney cells, serum-free

Cell Line Description

Vero cell line (Sigma catalog no. 84113001) adapted to grow in animal component free medium (VP-SFM; Gibco catalog no. 11681-020).

Culture Medium

VP-SFM (Gibco cat no. 11681-020) + 4 mM L-Glutamine (G7513). When freezing cells down use culture medium (50:50 conditioned medium:fresh medium) + 10% DMSO (D2650).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:6, i.e., seeding at 1-3x10,000 cells/cm2 using 0.05% trypsin or trypsin/EDTA; 5% CO2; 37 °C.

Other Notes

Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.

Depositor and originator: Dr. E. Penn, European Collection of Cell Cultures, Health Protection Agency, Porton Down, Salisbury,

Safety & Documentation

Safety Information

WGK Germany 
3

Documents

Certificate of Analysis (COA)

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