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Sigma

108CC15

08062516

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Properties

Related Categories 0-10, Cell Culture, ECACC Cell Lines More...
biological source   Mouse x Rat Hybridoma nerve
growth mode   Adherent
karyotype   Modal No. varied with passage see reference
morphology   Neuronal
products   Long processes after treatment with dibutyryl cAMP, dense core vesicles, excitable membranes, neurotransmitter enzymes, synthesis of neurohormones and uptake of catecholamines.
receptors   Receptors for neurohormones: delta opioid, PGE1 vasoactive intestinal polypeptide, adenosine, somatostatin and acetylcholine.
application(s)   cell culture | mammalian: suitable
relevant disease(s)   cancer
shipped in   dry ice
storage temp.   −196°C

Description

Cell Line Origin

Mouse neuroblastoma x Rat glioma hybrid

Cell Line Description

The cell line108CC15 is formed by the fusion of mouse neuroblastoma N18TG2 and rat glioma C6-BU-1using inactivated Sendai virus. This cell line was renamed NG108-15 by Klee and Nirenberg. Sigma has two catalogue entries for the 108CC15 cell line which differ in that they were deposited by different groups: 108CC15 from the original group that derived the cell line (Sigma Catalogue number 08062516) and NG108-15 (Sigma Catalogue number 88112302). Several other cell lines in the Sigma catalogue have been derived from 108CC15. This is a cholinergic hybrid cell line.

Application

Used as a neuronal Model

Culture Medium

DMEM + 2 mM glutamine + 10% Foetal Bovine Serum (FBS) HAT (0.1 mM hypoxanthine, 10 μM aminopterin and 16 μM thymidine) Cells can be cultured without HAT provided they were cultured inbetween in HT medium for 3-4 days

Subculture Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 1-2x10,000 cells/cm2 using 0.05% trypsin/EDTA; 5% CO2; 37°C. The cultures rapidly produce lactic acid through anaerobic glycolysis causing the media to become acidified. The medium must never be allowed to become acidic as cells detach in clumps and take many passages to recover. Frequent inspection of cultures is therefore required.

Other Notes

Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.

Depositor and originator: Professor Bernd Hamprecht, Interfaculty Institute for Biochemistry, University of Tuebingen, Hoppe-Seyler Street 4, 72076 Tuebingen, Germany.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis (COA)

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