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Sigma

UM-UC-7

08090504

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Properties

Related Categories Cell Culture, ECACC Cell Lines, S-Z More...
biological source   human bladder
growth mode   Adherent
karyotype   Modal number of chromosomes is 70
morphology   Epithelial
products   Not specified
receptors   Not specified
application(s)   cell culture | mammalian: suitable
relevant disease(s)   cancer
shipped in   dry ice
storage temp.   −196°C

Description

Cell Line Origin

Human transitional cell carcinoma of the bladder

Cell Line Description

UM-UC-7 was obtained from an open operative specimen from a transitional carcinoma of the bladder in a male patient. UM-UC-7 was found to produce tumours in athymic nude mice.

DNA Profile

STR-PCR Data:
Amelogenin: X,Y
CSF1PO: 10
D13S317: 12
D16S539: 10,11
D5S818: 12
D7S820: 10,11
THO1: 9
TPOX: 8,11
vWA: 16

Culture Medium

EMEM (EBSS) + 2 mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Fetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:6, i.e., seeding at 2-4x10,000 cells/cm2 using 0.05% trypsin/EDTA; 5% CO2; 37 °C. Doubling time approximately 19 hours.

Other Notes

Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.

Depositor and originator: Dr H B Grossman Department of Urology, University of Texas, M.D. Anderson Cancer Centre, 1515 Holcombe Boulevard, Unit 1373, Houston, Texas, 77030 USA

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis

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Cell Culture Guide

ECACC Laboratory Handbook
Protocols & Articles
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