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Sigma

UM-RC-3

08090512

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Properties

Related Categories Cell Culture, ECACC Cell Lines, S-Z More...
biological source   human kidney (renal gland)
growth mode   Adherent
karyotype   Median number of chromosomes is 55
morphology   Polygonal
products   Not specified
receptors   Not specified
application(s)   cell culture | mammalian: suitable
relevant disease(s)   cancer
shipped in   dry ice
storage temp.   −196°C

Description

Other Notes

Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.

Depositor and originator: Dr H B Grossman Department of Urology, University of Texas, M.D. Anderson Cancer Centre, 1515 Holcombe Boulevard, Unit 1373, Houston, Texas, 77030 USA

Application

Study of tumour associated antigens.

Cell Line Origin

Human renal carcinoma

DNA Profile

STR-PCR Data: Amelogenin: X
CSF1PO: 12
D13S317: 9,12
D16S539: 9
D5S818: 9,12
D7S820: 9
THO1: 9.3
TPOX: 8
vWA: 16,18

Culture Medium

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 1-2x10,000 cells/cm2 using 0.05% trypsin/EDTA; 5% CO2; 37°C. Doubling time approximately 29 hours.

Cell Line Description

UM-RC-3 is a continuous cell line established from a primary renal adenocarcinoma. It exhibits a loss of contact inhibition and produces tumours in the nude mouse model. The patient had metastatic disease at the time of their nephrectomy.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis


Cell Culture Guide

ECACC Laboratory Handbook
Protocols & Articles
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