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Sigma

1.2B4

10070103, from human pancreas(islets)

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Properties

Related Categories 0-10, Cell Culture, ECACC Cell Lines More...
biological source   human pancreas (islets)
growth mode   Adherent
karyotype   Modal chromosome number 67-71
morphology   Epitheloid
products   1.2B4 cells have been shown to express insulin, glucokinase and IAPP by immunocytochemistry. These cells express the GLUT1 glucose transporter.
receptors   Not specified
application(s)   cell culture | mammalian: suitable
shipped in   dry ice

Description

Cell Line Description

1.2B4 is a hybrid cell line formed by the electrofusion of a primary culture of human pancreatic islets with HuP-T3, a human pancreatic carcinoma cell line (Sigma catalog number 93121055). 1.2B4 has been shown to be tumourigenic when transplanted into a SCID mouse host. The cell line has applications in the study of pancreatic cell biology. 1.2B4 cells provide a method of producing pure insulin secreting cells. This is an alternative to the use of primary tissue in cell transplantation therapies for type 1 diabetes.

DNA Profile

STR-PCR Data:
Amelogenin: X,Y
CSF1PO: 10
D13S317: 9,11
D16S539: 10,13
D5S818: 10,14
D7S820: 12
THO1: 9
TPOX: 8,12
vWA: 18

Culture Medium

RPMI-1640 + 2 mM Glutamine + 10% FCS / FBS

Subculture Routine

Split sub-confluent cultures (70-80%) 1:2 to 1:6 , i.e., seeding at 2-4 x 104 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37 °C. Population doubling approx 20hrs. At confluence 105 cells/cm2 can be expected.

Other Notes

Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.

Legal Information

Flatt P & McClenaghan N (2004) Insulin producing cell-line US patent number 20040005702 (A1). Also WO0210346 (A2).

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis (COA)

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Protocols & Articles
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