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Sigma

1-7HB2

10081201, from human breast

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Properties

Related Categories 0-10, Cell Culture, ECACC Cell Lines More...
biological source   human breast
growth mode   Adherent
karyotype   Not specified
morphology   Cuboidal
products   Formation of branching structures in the presence of fibroblast cultures or fibroblast-conditioned medium. Formation of spheroids in collagen type I gels.
receptors   Not specified
application(s)   cell culture | mammalian: suitable
relevant disease(s)   cancer
shipped in   dry ice

Description

Cell Line Origin

Breast cancer, morphology, adhesion properties

Cell Line Description

1-7HB2 is a clonal derivative of the human mammary luminal epithelial cell line MTSV1-7 identified by screening for branching morphology. The MTSV1-7 is a clonal non-tumourigenic cell line developed by immortalizing luminal epithelial cells cultured from milk using the SV40 T antigen. (See also MTSV1-7 CE1 Sigma catalog number 10081202.). Used for in vitro study of branching morphogenesis of human mammary epithelial cells and the role of integrins in mammary morphogenesis.

DNA Profile

STR-PCR Data:
Amelogenin: X
CSF1PO: 12,13
D13S317: 12
D16S539: 8,13
D5S818: 10,13
D7S820: 8,10
THO1: 7,9
TPOX: 11
vWA: 14,17

Culture Medium

DMEM + 2 mM Glutamine + 10% Fetal Bovine Serum (FBS) + Hydrocortisone (5 μg/ml) + Bovine Insulin (10 μg/ml).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:4 to 1:10 seeding at 3 x 104 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37 °C.

Other Notes

Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis (COA)

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Pipette Controller from Brand
Protocols & Articles
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