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Sigma

TO14

from human ovary, 10081203

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Properties

Related Categories Cell Culture, ECACC Cell Lines, S-Z More...
biological source   human ovary
growth mode   Adherent
karyotype   For modal chromosome numbers see Langdon SP et.al. (1988) Cancer Research Nov 1; 48(21):6166-72.
morphology   Epithelial; stellate in shape and producing hemicystic colonies which grow upwards from the substrate and detach as spheres.
products   Oestrogen receptor negative cell line.
receptors   Oestrogen receptor negative cell line. For antigen expression see Langdon SP et.al. (1988) Cancer Research Nov 1; 48(21):6166-72.
application(s)   cell culture | mammalian: suitable
relevant disease(s)   metastasis
shipped in   dry ice

Description

Other Notes

Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.

Cell Line Origin

Human ovarian cancer; oestrogen receptor negative, drug efficacy and toxicity

DNA Profile

Not specified

Culture Medium

RPMI 1640 + 2 mM Glutamine + 2 mM Sodium Pyruvate + 10% Fetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:4 to 1:10 seeding at 2- 3 x 104 cells/cm2<sup/>

Cell Line Description

TO14 is an adherent cell line derived from a metastasis to the omentum obtained at hysterectomy from a patient with a well differentiated serous adenocarcinoma. TO14 exhibits poor growth in semi-solid medium (agar). TO14 is from the same patient as PEO23 and PEO14 cell lines (Sigma catalog numbers 10032313 and 10032311 respectively).

This cell line is one of nine from the PE ovarian adenocarcinoma panel (derived from 4 patients at varying stages of ovarian cancer, isolated from various malignant sites, and at various treatment stages) available at ECACC which provides a model system for research into the mechanism of oestrogen action on ovarian adenocarcinoma tumour cells, and for the study of efficacy and toxicity of oestrogen protagonists.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis


Cell Culture Guide

ECACC Laboratory Handbook
Protocols & Articles
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